Effects of Drying Methods and Extraction Conditions on Total Phenolic and Flavonoid content and Antioxidant Activities of Helicteres isora L. Fruit Extracts
Abstract
This research studied effects of drying methods and extraction conditions on total phenolic and flavonoid content and antioxidant activities of Helicteres isora L. extracts by testing three antioxidant activities were DPPH radical scavenging activity, reducing power and metal chelating activity. The extracts from overexposuring to the sun and drying at 170 oC exhibited the highest total phenolic content of all solvent extracts, excepted 95% ethanol extract. The extracts from drying at 150 oC exhibited the highest flavonoid content in ethanol extract. The flavonoid content of aqueous extracts were depended on drying temperature. To determine antioxidant activities, reducing power and DPPH radical scavenging activity increased when the drying temperature was increased. The total phenolic content also showed good influence to antioxidant activities, reducing power and DPPH radical scavenging activity as evidenced by the correlation coefficient value of 0.9785 and 0.8115. All 70% ethanol extracts showed the highest metal chelating activity. The metal chelating activity also showed moderate correlation to the total phenolic content but very low correlations to total flavonoid content.Keywords : total phenolic content, flavonoid content, antioxidant, Helicteresisora L.References
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Menke, A., Casagrande, S., Geiss, L., &Cowie, C. C. (2015). Prevalence of and trends in diabetes among adults in the United States, 1988-2012. The Journal of the American Medical Association, 314(10), 1021-1029.
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Prajapathi, N. D., Purohit, S. S., Sharmi, A. K., & Kumar, T. (2003). A Handbook of Medicinal Plants: A Complete Source Book. Jodhpur India: Shyam Printing Press.
Ramful, D., Tarnus, E., Aruoma, O. I., Bourdon, E., &Bahorun, T. (2011). Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps. Food Research International, 44, 2088–2099.
Shori, A. (2013). An emerging medicinal plant with promising in vitro antioxidant potential Helicteresisora Linn. In International Conference and Exhibition onPharmacognosyPhytochemistry& Natural Products. Hyderabad India: Omics Group.
Srisook, K., Salee, P., Charoensuk, Y., &Srisook, E. (2010). In vitro anti-oxidant and anti-tyrosinase activities of the rhizomal extracts from Amomum biflorum Jack. Thai Journal of Botany, 2 (Special Issue), 143-150. (in Thai)
Wattanakul, U., Wattanakul, W., Lerssuthichawal, T., &Puengyam, P. (2009).Determination of antioxidant activity,total phenolic compounds and flavonoid contents of mangrove plants extracts from Rajamangala beach, Trang province. TSU Conference 19. Songkla: Thaksin University.(in Thai)
Xu, G. H., Ye, X. G., Chen, J. C., & Liu, D. H. (2007). Effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract. Journal of Agricultural and Food Chemistry, 55, 330–335.
Jain, M., Nilsson, R., Sharma, S., Madhusudhan, N., Kitami, T., Souza, A. L., Kafri, R., Kirschner, M. W., Clish, C. B., &Mootha, V. K. (2012). Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science Translational Medicine, 336(6084), 1040-1044.
Jain, A., Sinha, P., & Desai, N. S. (2014). Estimation of flavonoid, phenol content and antioxidant potential of Indian screw tree (Helicteresisora L.). International Journal of Pharmaceutical Sciences and Research, 5(4),1320-1330.
Jeong, S. M., Kim, S. Y., Kim, D. R., Jo, S. C., Nam, K. C., Ahn, D. U., & Lee, S. C. (2004). Effect of heat treatment on the antioxidant activity of extracts from citrus peels. Journal of Agricultural and Food Chemistry, 52, 3389-3393.
Khambai, M., Hasee, P., Chaiphosri, C., Liangraksa, V., Tinrat, S., &Singhapol, C. (2015). Antioxidant analysis of Garcinia cowa and East Indian screw tree extracted by different solvents. In Thailand-Japan Student Science Fair 2015. Bangkok: King Mongkut's University of Technology North Bangkok.
Kumar, V., Sharma, M., Lemos, M., &Shriram, V. (2013). Efficacy of Helicteresisora L. against free radicals, lipid peroxidation, protein oxidation and DNA damage. Journal of Pharmacy Research, 6, 620-625.
Lou, S. N., Lin, Y. S., Hsu, Y. S., Chiu, E. M., & Ho, C. T. (2014). Soluble and insoluble phenolic compounds and antioxidant activity of immature calamondin affected by solvents and heat treatment. Food Chemistry, 161, 246-253.
Menke, A., Casagrande, S., Geiss, L., &Cowie, C. C. (2015). Prevalence of and trends in diabetes among adults in the United States, 1988-2012. The Journal of the American Medical Association, 314(10), 1021-1029.
Narita, Y., & Inouye, K. (2012). High antioxidant activity of coffee silverskin extracts obtained by the treatment of coffee silverskin with subcritical water. Food Chemistry, 135, 943–949.
Prajapathi, N. D., Purohit, S. S., Sharmi, A. K., & Kumar, T. (2003). A Handbook of Medicinal Plants: A Complete Source Book. Jodhpur India: Shyam Printing Press.
Ramful, D., Tarnus, E., Aruoma, O. I., Bourdon, E., &Bahorun, T. (2011). Polyphenol composition, vitamin C content and antioxidant capacity of Mauritian citrus fruit pulps. Food Research International, 44, 2088–2099.
Shori, A. (2013). An emerging medicinal plant with promising in vitro antioxidant potential Helicteresisora Linn. In International Conference and Exhibition onPharmacognosyPhytochemistry& Natural Products. Hyderabad India: Omics Group.
Srisook, K., Salee, P., Charoensuk, Y., &Srisook, E. (2010). In vitro anti-oxidant and anti-tyrosinase activities of the rhizomal extracts from Amomum biflorum Jack. Thai Journal of Botany, 2 (Special Issue), 143-150. (in Thai)
Wattanakul, U., Wattanakul, W., Lerssuthichawal, T., &Puengyam, P. (2009).Determination of antioxidant activity,total phenolic compounds and flavonoid contents of mangrove plants extracts from Rajamangala beach, Trang province. TSU Conference 19. Songkla: Thaksin University.(in Thai)
Xu, G. H., Ye, X. G., Chen, J. C., & Liu, D. H. (2007). Effect of heat treatment on the phenolic compounds and antioxidant capacity of citrus peel extract. Journal of Agricultural and Food Chemistry, 55, 330–335.
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2017-01-31
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