Population Genetic Structure of Wing Shell (Strombus canarium) along the Andaman Sea Coast
Abstract
Wing shell (Strombus canarium) is an important commercial fishery product in Southern Thailand. Due to dramatic decreases in S. canarium populations, caused by overexploitation, an effective sustainable management strategy is needed. This plan needs to be based on detailed information of genetic features. In this study, population genetic structure and demographic history of the wing shell (Strombus canarium) living along the Andaman Sea coast was analyzed based on the variation of the nucleotide sequence (497 bp) of mitochondrial DNA in cytochrome oxidase subunit I. The mtDNA sequences of 84 individual collected from 5 sampling sites: Satun, Trang, Krabi, Phuket and Phang Nga province, were analyzed. In total, 17 haplotypes were identified. Estimated values of haplotype and nucleotide diversities were 0.762 and 0.002, respectively. Neutrality tests (Tajima's D and Fu's FS statistics) showed that Andaman populations of S. canarium had experienced expansion. In addition, the analysis of mismatch distribution accepted the sudden expansion model. The approximate time of the expansion was 100,000 years ago during the Pleistocene period. The topology of a minimum spanning network (MSN) was a star topology and did not indicate any distinct pattern of phylogeographic structure. An AMOVA analysis showed no genetic structure among populations. The analysis of pairwise differences (FST) also showed no statistically significant difference between all possible regional combinations. Based on these results, an absence of a population structure of S. canarium on the Andaman Sea coast was possibly caused by a high level of gene flow. The results revealed by this study are necessary information contributing to efficient strategies to conserve this species in Andaman Sea. Keywords : mitochondrial DNA, genetic diversity, demographic history, Wing shell, Andaman Sea.References
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Brante, A., Fernández, M. & Viard, F. (2012). Phylogeography and biogeography concordance in the marine
Gastropod Crepipatella dilatata (Calyptraeidae) along the southeastern Pacific coast.
Journal of Heredity, 103 (5), 630-637.
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Cob, Z. C., Arshad, A., Idris, M. H., Bujang, J. S. & Ghaffar, M. A. (2008). Sexual polymorphism in a population of Strombus canarium Linnaeus, 1758 (Mollusca: Gastropoda) at Merambong Shoal, Malaysia. Zoological Studies, 47(3), 318-325.
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Fu, F. X. (1997). Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 14, 915-925.
Gu, Q H., Husemann, M., Ding, B., Luo, Z & Xiong, B. X. (2015). Population genetic structure of Bellamya
aeruginosa (Mollusca: Gastropoda: Viviparidae) in China: weak divergence across large geographic distances. Ecology and Evolution, 5(21), 4906-4919.
Harpending, R. C. (1994). Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution.Human Biology, 66, 591-600.
Huang, X. & Madan, A. (1999). CAP3: a DNA sequence assembly program. Genome Research, 9, 868-877.
Kyle, C. J. & Boulding, E.G. (2000). Comparative population genetic structure of marine gastropods
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Pongparadon, S., Zuccarello, G. C., Phang, S., Kawai, H., Hanyudaand, T. & Prathep, A. (2015). Diversity of Halimeda (Chlorophyta) from the Thai-Malay Peninsula. Phycologia, 54 (4), 349-366.
Ramirez-Soriano A., Ramos-Onsins, S.E., Rozas, J. F. & Navarro, A. (2008). Statistical power analysis of neutrality tests under demographic expansions, contractions and bottlenecks with recombination. Genetics, 179, 555-567.
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Slatkin, M. & Hudson, R. R. (1991). Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations. Genetics, 129, 555-562.
Supmee, V., Ngernsiri, L., Sriboonlert, A., Wonnapinij , P. & Sangthong, P. (2012) Population genetic analysis of Violet vinegar crab (Episesarma versicolor) along the Andaman sea coast of Thailand, Zoological Studies, 51(7), 1040-1050.
Supmee, V. (2015). Population genetic structure and demographic history of Spotted scat (Scatophagus argus) in
Southern of Thailand. Phranakhon Rajabhat Research Journal, 10 (2), 38-56. (in Thai)
Soonsun, P., Detsathit, S., Chairut, P. & Jirawuttho, S. (2007). Studies on Spawning Stimulation, Embryonic and
Larval Development of Dog Conch (Strombus canarium Linnaeus, 1758). Technical Paper No. 8/2007. Krabi Coastal Fisheries Research and Development Center, Coastal Fisheries Research and Development Bureau, Department of Fisheries. (in Thai)
Suppapan, J. (2015). Population genetic structure of Greenback Mullet (Liza subviridis) in Gulf of Thailand :
Implication for conservation. Phranakhon Rajabhat Research Journal, 10 (1), 118-130. (in Thai)
Tajima, F. (1989). Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, 123, 585–595.
Thongnunui, P., Tanyaros, S., Sudtongkong, C., Songrak, A., Sroytong, A., Janyong, S., Anantasuk, R.,
Intacharoen, P. & Kongprom. (2009). Status and Management Concept for Sustainability of Hard Clam, Meretrix casta and Wing Shell, Strombus canarium in the Coastal Area of Trang Province. Annual Research Report (2008 - 2009). Faculty of Fishery Science and Technology, Rajamangala University of Technology Srivijaya. (in Thai)
Watterson, G. A. (1984). Allele frequencies after a bottleneck. Theoretical Population Biology, 26, 387-407.
Xu, Q., Liu, R. & Liu, Y. (2009). Genetic population structure of the swimming crab, Portunus trituberculatus in the East China Sea based on mtDNA 16S rRNA sequences. Journal of Experimental Marine Biology and Ecology, 371, 121-129.
Xue D.X., Wang, H. Y., Zhang, T. & Liu, J.X. (2014). Population Genetic Structure and Demographic History of
Atrina pectinata Based on Mitochondrial DNA and Microsatellite Markers. PLoS ONE. 9(4), e95436. doi:10.1371/journal.pone.0095436.
Yang, Z. (2006). Computational molecular evolution. Oxford University Press, New York.
(1996-2000) of the west coast of Thailand, Andaman Sea. Phuket Marine Biological Center Research Bulletin, 63, 53-76.
Boore, J. L. (1999). Survey and summary animal mitochondria genome. Nucleic acids research, 27(8),1767-1780.
Brante, A., Fernández, M. & Viard, F. (2012). Phylogeography and biogeography concordance in the marine
Gastropod Crepipatella dilatata (Calyptraeidae) along the southeastern Pacific coast.
Journal of Heredity, 103 (5), 630-637.
Chiu, Y., Bor, H., Tan, M., Lin, H. & Jean, C. (2013). Phylogeography and genetic differentiation among populations of the Moon Turban Snail Lunella granulate Gmelin, 1791 (Gastropoda: Turbinidae). International Journal of Molecular Sciences, 14, 9062-9079.
Cob, Z. C., Arshad, A., Idris, M. H., Bujang, J. S. & Ghaffar, M. A. (2008). Sexual polymorphism in a population of Strombus canarium Linnaeus, 1758 (Mollusca: Gastropoda) at Merambong Shoal, Malaysia. Zoological Studies, 47(3), 318-325.
Cob, Z. C., Arshad, A., Ghaffar, M. A., Bujang, J. S., & Muda, W. L. W. (2009). Development and growth of larvae of the Dog Conch, Strombus canarium (Mollusca: Gastropoda), in the laboratory. Zoological Studies, 48 (1), 1-11.
Davis, M., Bolton, C.A. & Stoner, A.W. (1993). A comparison of larval development, growth, and shell morphology in three Caribbean Strombus species. Veliger, 36, 236-244.
Excoffier, L. & Lischer, H. E. L. (2010). Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, 564-567.
Fu, F. X. (1997). Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 14, 915-925.
Gu, Q H., Husemann, M., Ding, B., Luo, Z & Xiong, B. X. (2015). Population genetic structure of Bellamya
aeruginosa (Mollusca: Gastropoda: Viviparidae) in China: weak divergence across large geographic distances. Ecology and Evolution, 5(21), 4906-4919.
Harpending, R. C. (1994). Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution.Human Biology, 66, 591-600.
Huang, X. & Madan, A. (1999). CAP3: a DNA sequence assembly program. Genome Research, 9, 868-877.
Kyle, C. J. & Boulding, E.G. (2000). Comparative population genetic structure of marine gastropods
(Littorina spp.) with and without pelagic larval dispersal. Marine Biology,137, 835-845.
Larkin, M. A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007). Sequence analysis Clustal W and Clustal X version 2.0. Bioinformatics Applications Note, 23 (21), 2947-2948.
Latiolais, J.M., Taylor, M.S., Roy, K. & Hellberg, M.E. (2006). A molecular phylogenetic analysis of strombid gastropod morphological diversity. Molecular Phylogenetics and Evolution, 41, 436-444.
Librado, P. & Rozas, J. (2009). DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451-1452.
Nei, M. (1987). Molecular Evolutionary Genetics. New York : Columbia University Press.
Planes, S., Doherty, P.J. & Bernardi, G. (2001). Strong genetic divergence among populations of a marine fish with limited dispersal, Acanthochromis polyacanthus, within the great barrier reef and the coral sea. Evolution, 55 , 2263-2273.
Pongparadon, S., Zuccarello, G. C., Phang, S., Kawai, H., Hanyudaand, T. & Prathep, A. (2015). Diversity of Halimeda (Chlorophyta) from the Thai-Malay Peninsula. Phycologia, 54 (4), 349-366.
Ramirez-Soriano A., Ramos-Onsins, S.E., Rozas, J. F. & Navarro, A. (2008). Statistical power analysis of neutrality tests under demographic expansions, contractions and bottlenecks with recombination. Genetics, 179, 555-567.
Sinsakul, S. (2000). Late Quaternary geology of the Lower Central Plain, Thailand. Journal of Southeast Asian.
Earth Science, 18, 415-426.
Slatkin, M. & Hudson, R. R. (1991). Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations. Genetics, 129, 555-562.
Supmee, V., Ngernsiri, L., Sriboonlert, A., Wonnapinij , P. & Sangthong, P. (2012) Population genetic analysis of Violet vinegar crab (Episesarma versicolor) along the Andaman sea coast of Thailand, Zoological Studies, 51(7), 1040-1050.
Supmee, V. (2015). Population genetic structure and demographic history of Spotted scat (Scatophagus argus) in
Southern of Thailand. Phranakhon Rajabhat Research Journal, 10 (2), 38-56. (in Thai)
Soonsun, P., Detsathit, S., Chairut, P. & Jirawuttho, S. (2007). Studies on Spawning Stimulation, Embryonic and
Larval Development of Dog Conch (Strombus canarium Linnaeus, 1758). Technical Paper No. 8/2007. Krabi Coastal Fisheries Research and Development Center, Coastal Fisheries Research and Development Bureau, Department of Fisheries. (in Thai)
Suppapan, J. (2015). Population genetic structure of Greenback Mullet (Liza subviridis) in Gulf of Thailand :
Implication for conservation. Phranakhon Rajabhat Research Journal, 10 (1), 118-130. (in Thai)
Tajima, F. (1989). Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, 123, 585–595.
Thongnunui, P., Tanyaros, S., Sudtongkong, C., Songrak, A., Sroytong, A., Janyong, S., Anantasuk, R.,
Intacharoen, P. & Kongprom. (2009). Status and Management Concept for Sustainability of Hard Clam, Meretrix casta and Wing Shell, Strombus canarium in the Coastal Area of Trang Province. Annual Research Report (2008 - 2009). Faculty of Fishery Science and Technology, Rajamangala University of Technology Srivijaya. (in Thai)
Watterson, G. A. (1984). Allele frequencies after a bottleneck. Theoretical Population Biology, 26, 387-407.
Xu, Q., Liu, R. & Liu, Y. (2009). Genetic population structure of the swimming crab, Portunus trituberculatus in the East China Sea based on mtDNA 16S rRNA sequences. Journal of Experimental Marine Biology and Ecology, 371, 121-129.
Xue D.X., Wang, H. Y., Zhang, T. & Liu, J.X. (2014). Population Genetic Structure and Demographic History of
Atrina pectinata Based on Mitochondrial DNA and Microsatellite Markers. PLoS ONE. 9(4), e95436. doi:10.1371/journal.pone.0095436.
Yang, Z. (2006). Computational molecular evolution. Oxford University Press, New York.
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2016-10-10
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