Isolation and Anti-biofilm Activity of Lytic Phages against Pseudomonas aeruginosa TISTR1287

Authors

  • Nanthanit Jaruseranee School of Science, Mae Fah Luang University
  • Srinuan Niyom Microbial Products and Innovation Research Unit, Mae Fah Luang University
  • Somboon Kamtaeja Faculty of Education, Chiang Rai Rajabhat University

Abstract

Lytic phages act as an alternative inhibitor against  Pseudomonas aeruginosa  biofilms. A biofilm, a matrix composed of bacterial extracellular polymeric substances, is vital in exacerbating the spread of antibiotic resistance. This research aimed to isolate lytic phages from water and soil samples collected from Mae Fah Luang University premises and investigate their anti-biofilm activity against P. aeruginosa TISTR1287. Phages were isolated using an enrichment protocol and a double overlay agar plaque assay. Four lytic phage isolates were identified and named PAMFUP1, PAMFUP2, PAMFUP3, and PAMFUP4. Spot tests revealed that all four phages are specific for P. aeruginosa TISTR1287. The plaque diameters reached by phage isolates PAMFUP1, PAMFUP2, PAMFUP3, and PAMFUP4 were 0.80±0.15 mm, 0.77±0.18 mm, 0.98±0.13 mm, and 3.90±0.44 mm, respectively. Finally, crystal violet assays were performed to determine biofilm inhibition by four phages. The results demonstrated that these four phages significantly reduced the biofilm formation at 8 hrs and 24 hrs post incubation (p<0.00).

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Published

2023-09-25