Genetic Diversity and Phylogenetic Relationships of Ball Sea Cucumber (Phyllophorella kohkutiensis) in Thai Waters

Authors

  • Thadsin Panithanarak

Abstract

Ball sea cucumbers (Phyllophorella kohkutiensis) were originally found in Thailand. They have been increasingly fishing from both the Gulf of Thailand and the Andaman Sea. Nevertheless, basic knowledge essentially required for the species management has never been reported in Thailand. This study aimed to investigate genetic diversity, confirm the species identification and examine phylogenetic relationships of ball sea cucumbers in Thai waters, by analyzing DNA sequence variation at cytochrome c oxidase subunit I in mitochondrial DNA. The results suggested that ball sea cucumbers from Tumbon Koh Sarai, Amphur Muang, Satun Province (N = 15), Ban Don Bay, Amphur Tha Chang, Surat Thani Province (N = 15) and Koh Chang, Trat Province (N = 2) showed high haplotype diversity (h = 0.675 ± 0.089) but their nucleotide diversity was low (π = 0.00192 ± 0.00054). All ball sea cucumbers were the same species which was Phyllophorella kohkutiensis. Pairwise genetic distances between sea cucumber samples (distance measure = Kimura 2-parameter) were ranging from 0-1.07%. This is consistent with phylogenetic analysis between ball sea cucumbers and other sea cucumber species. Phylogenetic trees based on maximum parsimony and neighbor-joining methods showed that ball sea cucumbers were clustered within a single clade.                                Keywords :  ball sea cucumber ; Phyllophorella kohkutiensis ; Thai waters ; COI      

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Published

2021-05-05