Preparation of Fluorescent Carbon Dots Using Microwave-Assisted Pyrolysis and Its Application in Biolabeling
Abstract
In this work, we synthesized fluorescent carbon dots (CDs) from citric acid and urea using an easy and rapid method so called “microwave-assisted pyrolysis”. The synthesized CDs under the difference in microwave heating time provided different colors of fluorescent light. Under UV illumination, it was observed that the maximum intensity of blue fluorescence was carried out from microwave heating process at 5 min. Characterization of CDs was analyzed by UV-Vis absorption, fluorescent spectra, fourier-transform infrared (FTIR) and transmission electron microscope (TEM). The synthesized CDs were less than 5 nm in size and good in optical properties. Moreover, we investigated the use of CDs in biolabeling application by linking CDs with 8-hydroxy-2’-deoxyguanosine (8-OHdG) antibody using glutaraldehyde as molecular cross linker. This work can be applied to analysis and quantification of 8-OHdG, which is a biological marker indicating of oxidative stress in human body. Keywords : carbon dots (CDs) ; fluorescence ; microwave-assisted pyrolysis method ; antibody ; 8-OHdGReferences
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Dong, W., Zhou, S., Dong, Y., Wang, J., Ge, X., & Sui, L. (2015). The preparation of ethylenediamine-modified fluorescent carbon dots and their use in imaging of cells. Luminescence, 30(6), 867-871.
Li, H., He, X., Liu, Y., Huang, H., Lian, S., Lee, S.-T., & Kang, Z. (2011). One-step ultrasonic synthesis of water-soluble carbon nanoparticles with excellent photoluminescent properties. Carbon, 49(2),
605-609.
Li, M., Chen, T., Gooding, J. J., & Liu, J. (2019). Review of carbon and graphene quantum dots for sensing. ACS Sensors, 4(7), 1732-1748.
Li, N., Than, A., Wang, X., Xu, S., Sun, L., Duan, H., Xu, C., & Chen, P. (2016). Ultrasensitive profiling of metabolites using tyramine-functionalized graphene quantum dots. ACS Nano, 10(3), 3622-3629.
Liu, H., Zhao, X., Wang, F., Wang, Y., Guo, L., Mei, J., Tian, C., Yang, X., & Zhao, D. (2017). High-efficient excitation-independent blue luminescent carbon dots. Nanoscale Research Letters, 12(1), 399.
Liu, M. L., Chen, B. B., Li, C. M., & Huang, C. Z. (2019). Carbon dots: synthesis, formation mechanism, fluorescence origin and sensing applications. Green Chemistry, 21(3), 449-471.
Ma, J. L., Yin, B. C., Wu, X., & Ye, B. C. (2017). Simple and cost-effective glucose detection based on carbon nanodots supported on silver nanoparticles. Analytical Chemistry, 89(2), 1323-1328.
Miao, H., Wang, L., Zhuo, Y., Zhou, Z., & Yang, X. (2016). Label-free fluorimetric detection of CEA using carbon dots derived from tomato juice. Biosensors and Bioelectronics, 86, 83-89.
Qu, F., Guo, X., Liu, D., Chen, G., & You, J. (2016). Dual-emission carbon nanodots as a ratiometric nanosensor for the detection of glucose and glucose oxidase. Sensors and Actuators B: Chemical, 233, 320-327.
Reyes, D., Camacho, M., Camacho, M., Mayorga, M., Weathers, D., Salamo, G., Wang, Z., & Neogi, A. (2016). Laser ablated carbon nanodots for light emission. Nanoscale Research Letters, 11, 424, 1-11.
Shi, Z., Yingzhe, Z., Xin, C., Fei, Z., Liyun, Z., Guangshuo, W., Jie, G., Yingying, M., Lianhong, Z., Bin, F., & Ruitao, Y. (2020). Mesoporous superparamagnetic cobalt ferrite nanoclusters: Synthesis, characterization and application in drug delivery. Journal of Magnetism and Magnetic Materials, 498, 166222.
Wang, C.-I., Wu, W.–C., Periasamy, A. P., & Chang, H.–T. (2014). Electrochemical synthesis of photoluminescent carbon nanodots from glycine for highly sensitive detection of hemoglobin. Green Chemistry, 16(5), 2509–2514.
Wu, Y., Wei, P. Pengpumkiat, S., Schumacher, E. A., & Remcho, V. T. (2015). Development of a carbon dot (C-Dot)-linked immunosorbent assay for the detection of human alpha-fetoprotein. Analytical Chemistry, 87(16), 8510-8516.
Xiao, N., Liu, S. G., Mo, S., Yang, Y. Z., Han, L., Ju, Y. J., Li, N. B. & Luo, H. Q. (2018). B,N-carbon dots-based ratiometric fluorescent and colorimetric dual-readout sensor for H2O2 and H2O2-involved metabolites detection using ZnFe2O4 magnetic microspheres as peroxidase mimics. Sensors and Actuators B: Chemical, 273, 1735-1743.
Xu, X., Ray, R., Gu, Y., Ploehn, H. J., Gearheart, L., Raker, K., & Scrivens, W. A. (2004). Electrophoretic analysis and purification of fluorescent single-walled carbon nanotube fragments. Journal of the American Chemical Society, 126(40), 12736-12737.
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2021-05-05
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