The Standard Karyotypes and Idiograms of Rose Danio (Danio roseus) and Laos Danio (Devario laoensis)
Abstract
The Rosy Danio (Danio roseus) and Laos Danio (Devario laoensis) are commonly found is small stream in Phayao province. The description of karyotype of fish is basic information for explaining the fish evolution and applying to further study in genetics. In this study we investigate the standardized karyotype and idiogram of Da. roseus and De. laoensis. Both fishes were collected from the tributary of Ing River and carried to University of Phayao. The fish metaphase chromosomes were directly prepared from the kidney of ten specimens per species (five males and five females). After chromosomal preparation, the chromosomes were stained with simple Giemsa’s staining method. The results showed that the diploid chromosome numbers (2n) of both fishes were 50, with the fundamental numbers (NF) being 100 and 96, respectively. Additionally, the karyotype of metacentric, submetacentric, acrocentric and telocentric chromosomes of Da. roseus and De. laoensis were 8-34-8-0 and 6-20-20-4, respectively. Surprisingly, the sex-chromosome dimorphism was not found in both fishes. Therefore, the karyotype formulas could be deduced as: Da. roseus; 2n (50) = Lm8 + Lsm16 + Msm18 + Ma8De. laoensis; 2n (50) = Lm2+ Lsm8+ La8 + Mm4 + Msm12 + Ma12 + Mt4 Keywords : Danio roseus ; Devario laoensis ; karyotype ; chromosome ; idiogramReferences
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Chaiyasan, P., Supiwong, W., Saenjundaeng, P., Seetapan, K., Pinmongkhonkul, S. & Tanomtong A. (2018). A Report on Classical Cytogenetics of Hihgfin Barb Fish, Cyclocheilichthys armatus (Cypriniformes, Cyprinidae). Cytologia 83(2), 149 – 154.
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Gomonteir, B., Tanomtong, A., Supiwong, W., Sikhruadong, S., Neeratanaphan, L. & Sanoamuang, L. (2012). Standardized karyotype and idiogram of two-spot glass catfish, Ompok bimaculatus (Siluriformes, Siluridae) in Thailand by conventional and Ag-NOR staining techniques. Cytologia, 77(4), 459 - 464.
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Saenjundaeng, P.,Cioffi, M. D. B.,Oliveira, E. A. D.,Tanomtong, A.,Supiwong, W.,Phimphan, S.,Pereira, M. J. C., Sember, A., Bertollo, L. A. C.,Liehr, T., Yano, C.F., Hatanaka, T. & Ráb, P. (2018a). Chromosomes of Asian cyprinid fishes: cytogenetic analysis of two representatives of small paleotetraploid tribe Probarbini. Molecular Cytogenetics 11(51),1 – 9.
Saenjundaeng, P., Kaewmad, P., Supiwong, W., Pinthong, K., Pengseng, P. & Tanomtong, A. (2018b).
Karyotype and Characteristics of Nucleolar Organizer Regions in Longfin Carp, Labiobarbus leptocheilus (Cypriniformes, Cyprinidae). Cytologia ,83(3), 265 – 269.
Sophawanus, M., Tanomthong, A., & Supiwong, W. (2017). Standardized Karyotype and Idiogram of Sicklefin Barb (Puntioplites falcifer) in Thailand. Koch. Cha. Sarn. Journal of Science, 39(2), 75 – 84. (in Thai)
Sukham, S., Chingakham, B., Thoidingjam, L. & Waikhom, G. (2013). Cytogenetic characterization of Devario aequipinnatus (McClelland, 1839) and Devario yuensis (Arunkumar and Tombi, 1998) (Cypriniformes: Cyprinidae) from Manipur, northeast India. Turkish Journal of Zoology, 37(6), 706 - 712.
Supiwong, W., Tanomtong, A., Supanuam, P., Jantarat, S., Khakhong, S. & Sanoamuang, L. (2012). A Discovery of Nucleolar Organizer Regions (NORs) Polymorphism and Karyological Analysis of Smithʼs Barb, Puntioplites proctozysron (Cypriniformes, Cyprinidae) in Thailand. Cytologia, 77(1), 35 – 42.
Tanomtong, A. (2011). Cytogenetics. Khon Kaen University Press. Khon Kaen. 387 pp. (in Thai)
Yeesaem, N., Jantarat, S. & Yeesin, P. (2019). Cytogenetic characterigation of Rasbora einthovenii in Sirindhorn peat swamp forest, Narathiwat province. Journal of Fisheries Technology Research, 13(2),58 - 68.
(in Thai)
Arai, R. (2011). Fish karyotypes: A check list. Springer. Japan.
Bhatnagar, A., Yadav, A. S. & Kamboj, N. (2014). Karyomorphology of Three Indian Major form Haryana, India. Journal of Fisheries Sciences, 8(2), 95-103
Chaiyasan, P., Supiwong, W., Saenjundaeng, P., Seetapan, K., Pinmongkhonkul, S. & Tanomtong A. (2018). A Report on Classical Cytogenetics of Hihgfin Barb Fish, Cyclocheilichthys armatus (Cypriniformes, Cyprinidae). Cytologia 83(2), 149 – 154.
Donsakul, T., Rangsiruji, A. & Magtoon, W. (2009). Karyotypes of five cyprinid fishes (Cyprinidae, Danioninae-Danionini): Rasbora agilis, R. dorsiocellata, R. rubrodorsalis, Boraras maculata and B. urophthalmoides from Thailand. In Proceedings of the 47th Kasetsart University Annual Conference. Kasetsart. Bangkok, Thailand. 320 - 327. (in Thai)
Fang, F. & Kottelat, M. (2000). Danio roseus, a new species from the Mekong basin in northeastern Thailand and northwestern Laos (Teleostei, Cyprinidae). Ichthyological Exploration of Freshwaters ,11(2),149-154.
Fang, F, Norén, M., Liao, T.Y, Källersjö, M., & Kullander, S.O. (2009). Molecular phylogenetic interrelationships of the south Asian cyprinid genera Danio, Devario and Microrasbora (Teleostei, Cyprinidae, Danioninae). Zoologica Scripta, 38, 237–256.
Galetti, P. M., Aguilar, C. T. & Molina, W. F. (2000). An overview of marine fish cytogenetics. Marine Genetics, 420, 55 - 62.
Gomonteir, B., Tanomtong, A., Supiwong, W., Sikhruadong, S., Neeratanaphan, L. & Sanoamuang, L. (2012). Standardized karyotype and idiogram of two-spot glass catfish, Ompok bimaculatus (Siluriformes, Siluridae) in Thailand by conventional and Ag-NOR staining techniques. Cytologia, 77(4), 459 - 464.
(in Thai)
Kottelat, M. (2001). Fishes of Laos. Gunaratne Offset Ltd., Sri lanka.
Kottelat, M. (2013). The fishes of the Inland Waters of Southeast Asia: A Catalogue and Core Bibliography of the Fishes Known to Occur in Freshwaters, Mangroves and Estuaries. The Raffles Bulletin of Zoology, 27,
1-663.
Kumar, R., Baisvar., V.S., Kushwaha, B., Waikhom, G. & Nagpure, N.S. (2017). Cytogenetic investigation of Cyprinus carpio (Linnaeus, 1758) using giemsa, silver nitrate, CMA3 staining and fluorescence in situ hybridization. Springer, 60, 1–8.
Na-Nakorn, U. (2000). Fish Genetics (2nd ed.). The Kasetsart University Press, Bangkok. 203 pp. (in Thai)
Neeratanaphan, L., Khamlerd, C., Chowrong, S., Intamat, S., Sriuttha, M. & Tengjaroenkul, B. (2017). Cytotoxic assessment of flying barb fish (Esomus metallicus) from a gold mine area with heavy metal contamination. International Journal of Environmental Studies ,74(4), 613–624.
Nelson, J.S. (2006). Fishes of the world. 4th ed. John Wiley and Son, Inc., New York.
Nelson, J.S., Grande, T.C., & Wilson, M.V.H. (2016). Fishes of the World. 5th Edition. John Wiley & Sons, Inc., Hoboken, New Jersey.
Nirchio, M., Turner, B. J., Perez, J. E., Gaviria, J. I. & Cequea, H. (2002). Karyotypes of three species of toadfish (Batrachoididae: Teleostei) from Venezuela. Scientia Marina, 66(1), 1 – 4.
Nithikulworawong, N. & Khrueanet, W. (2014). Karyotypic of Golden Little Barb, Puntius brevis Bleeker, 1850 (Pisces: Cyprinidae) from Khon Kaen Province. KKU Sci. J. 42(1), 106 – 118. (in Thai)
Panitvong, N. (2020). Freshwater Fishes of Thailand. Parbpim. Bangkok, Thailand. (in Thai)
Rainboth, W.J. (1996). Fishes of Cambodian Mekong. FAO Species Identification Field Guide for Fisheries Purpose. Mekong River Commission, FAO and DANIDA.
Saenjundaeng, P.,Cioffi, M. D. B.,Oliveira, E. A. D.,Tanomtong, A.,Supiwong, W.,Phimphan, S.,Pereira, M. J. C., Sember, A., Bertollo, L. A. C.,Liehr, T., Yano, C.F., Hatanaka, T. & Ráb, P. (2018a). Chromosomes of Asian cyprinid fishes: cytogenetic analysis of two representatives of small paleotetraploid tribe Probarbini. Molecular Cytogenetics 11(51),1 – 9.
Saenjundaeng, P., Kaewmad, P., Supiwong, W., Pinthong, K., Pengseng, P. & Tanomtong, A. (2018b).
Karyotype and Characteristics of Nucleolar Organizer Regions in Longfin Carp, Labiobarbus leptocheilus (Cypriniformes, Cyprinidae). Cytologia ,83(3), 265 – 269.
Sophawanus, M., Tanomthong, A., & Supiwong, W. (2017). Standardized Karyotype and Idiogram of Sicklefin Barb (Puntioplites falcifer) in Thailand. Koch. Cha. Sarn. Journal of Science, 39(2), 75 – 84. (in Thai)
Sukham, S., Chingakham, B., Thoidingjam, L. & Waikhom, G. (2013). Cytogenetic characterization of Devario aequipinnatus (McClelland, 1839) and Devario yuensis (Arunkumar and Tombi, 1998) (Cypriniformes: Cyprinidae) from Manipur, northeast India. Turkish Journal of Zoology, 37(6), 706 - 712.
Supiwong, W., Tanomtong, A., Supanuam, P., Jantarat, S., Khakhong, S. & Sanoamuang, L. (2012). A Discovery of Nucleolar Organizer Regions (NORs) Polymorphism and Karyological Analysis of Smithʼs Barb, Puntioplites proctozysron (Cypriniformes, Cyprinidae) in Thailand. Cytologia, 77(1), 35 – 42.
Tanomtong, A. (2011). Cytogenetics. Khon Kaen University Press. Khon Kaen. 387 pp. (in Thai)
Yeesaem, N., Jantarat, S. & Yeesin, P. (2019). Cytogenetic characterigation of Rasbora einthovenii in Sirindhorn peat swamp forest, Narathiwat province. Journal of Fisheries Technology Research, 13(2),58 - 68.
(in Thai)
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2021-05-05
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