Determination of synthetic dye by monolithic C18 HPLC

Authors

  • Kritsanapong Tongtip Department of Chemistry, Faculty of Science, Burapha University, Chonburi Thailand
  • Somsak Sirichai Department of Chemistry, Faculty of Science, Burapha University, Chonburi Thailand

Abstract

Method for determination of three synthetic dyes: Tartrazine, Amaranth and Indigo carmine by high-performance liquid chromatography was studied. The mobile phase was methanol: 20 mM ammonium acetate (pH 5.0) (15:85 v/v). The analytical column was a monolithic C18 (4.6 mm x 100 mm). The limits of detection (LOD) were in the range of 0.01 to 0.20 mg/L, and limit of quantification (LOQ) were in the range of 0.04 to 0.60 mg/L. The linearity and calibration curves with a correlation coefficient (R2) better than 0.9986 were obtained. Recoveries were in the range of 99.27–106.09 %.

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Published

2014-06-05

Issue

Vol. 19 (2014)

Section

Research Article