Effect of Cytokinins and Auxin on in vitro propagation of Fragaria x ananassa Duch. cv. Pharachatan 80
Abstract
The effect of plant growth regulators on in vitro propagation of Fragaria x ananassa Duch. cv. Pharachatan 80 was conducted in sterile conditions. For shoot multiplication, shoot tip were cultured on Murashige and Skoog (MS, 1962) medium for 6 weeks. The results indicated that shoot tips could induce shoots and roots 90%. Then shoot tips were cultured on MS medium supplemented with combination of cytokinin (BAP or Kinetin at 0, 0.1, 0.2 and 0.5 mg /l) and auxin (IBA at 0, 0.05, 0.1 and 0.2 mg/l) for 6 weeks. The results showed that shoot tips cultured on MS medium supplemented with 0.2 mg/l BAP and 0.2 mg/l IBA had the highest number of shoots (1.70 ± 0.82 shoots/explant) and shoots tips that cultured on MS medium supplemented with 0.2 mg/l IBA had the highest number of roots (5.40 ± 4.67 root/explant). From this study, it was found that MS medium supplemented with BAP and IBA gave the best results on shoot and root induction than MS medium supplemented with Kinetin and IBA. For the study of the survival percentage of strawberries obtained from tissue culture after transplanting for 4 weeks, it was found that the seedlings grown in the hydroponics system with the concentration of the main nutrient solution were 1/2 strength Hoagland's solution had the highest survival percentage (100%), root number, root length, leaves number and plant height above the ground than other planting methods. Keywords: shoot and root induction, Fragaria x ananassa Duch. cv. Pharachatan 80, cytokinin, auxinReferences
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Ministry of Agriculture and Cooperatives. (2003). Horticulture Tissue Technology. Bangkok: The Agricultural Cooperative Federation of Thailand. Limited. (in Thai)
Moradi, K., Otroshy, M., & Azimi, M.R. (2011). Micropropagation of strawberry by multiple shoots regeneration tissue cultures. Journal of Agricultural Technology, 7(6), 1755-1763.
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Phetkhong, S., Sumochitraporn, S., & Makkasab, C. (2010). Surface sterlization study and growth regulators
effect in tissue culture of aquatic plant Cryptocoryne albida. In technical paper No.9/2010. Pathumthani: Aquaculture animal genetics Research and Development Institute. (in Thai)
Phromkhun, A. (2001). Propagation of 4 varieties of strawberries of In Vitro Culture. Master of Science,
Program in Agricultural Technolog, Faculty of Science, Valaya Alongkorn Rajabhat University, Pathumthani. (in Thai)
Rency, A.S., Pandian, S., & Ramesh, M. (2018). Influence of adenine sulphate on multiple shoot induction in Clitoria ternatea L. and analysis of phyto-compounds in in vitro grown plants. Biocatalysis and Agricultural Biotechnology, 16, 181-191.
Samransuk, Y. (2002). The classification of strawberry varieties by DNA analysis method. Master of Science, Program in Biotechnology, Faculty of Science, Chiang Mai University, Chiang Mai. (in Thai)
Taghavi, S., Barac, T., Greenberg, B., Borremans, B., Vangronsveld, J., & Lelie, D. (2005). Horizontal Gene Transfer to Endogenous Endophytic Bacteria from Poplar Improves Phytoremediation of Toluene. Applied and environmental microbiology, 71(12), 8500-8505.
Techapinyawat, S. (2005). Plant physiology. Bangkok : Kasetsart University. (in Thai)
Thongaram, D. (2004). Hydroponics. Ratchaburi : Thammarak Printing. (in Thai)
Treftz, C., & Omaye, S.T. (2015). Comparision between hydroponic and soil systems for growing strawberries
in a greenhouse. International Journal of Agriculture Extension, 3(3), 195-200.
U-Kong, W., Chundet, R., & Buddharak, P. (2012). In vitro culture of Momordica charantia L. Thai Agriculture Research Journa, 30(2), 198-206. (in Thai)
Zayed, A., Lytle, C. M., Qian, J. Hong., & Terry, N. (1998). Chromium accumulation, translocation and
chemical speciation in vegetable crops. Planta, 206, 293-299
Beena, M. R., Martin, K. P., Kirti, P. B., & Hariharum, M. (2003). Rapid in vitro propagation of medicinally important Ceropegia candelabrum. Plant Cell, Tissue and Organ Culture, 72, 285-289.
Branca, C., Bucci, G., Domiano, P., Ricci, A., Torelli, A., & Bassi, M. (1991). Auxin structure and activity on tomato morphogenesis. Plant cell Tissue and Organ culture, 24(2), 105-114.
Cheruvathur, M. K., Abraham, J., & Thomas, D. (2015). In vitro micropropagation and flowering in Ipomoea sepiaria Roxb. An important ethanomedicinal plant. Asian Pacific Journal of Reproduction, 4(1), 49-53.
Daengsawat, C. (2001). Plant physiology. (2nd ed.). Bangkok: Development of education. (in Thai)
Deeying, S. (2007). Plant Growth Regulators. Bangkok: Faculty of Science and Technology Rajabhat Rajanagarindra University. (in Thai)
Eadrelder, A. M. (2013). An Effective Protocol for in vitro Storage and ex vitro Re-Growth of Strawberry Capsules. Journal of Chemistry & Biochemistry, 1(2), 30–38.
Harugade, S., Tabe, R.H., & Chaphalkar, S. (2014). Micropropagation of Strawberry (Fragaria X ananassa Duch.). International Journal of Current Microbiology and Applied Science, 3(3), 344-347.
Hasan, M.N., Nigar, S., Rabbi, M.A.K., Mizan, S.B., & Rahman, M.S. (2010). Micropropagation of Strawberry (Fragaria x ananassa Duch.). International Journal Sustain. Crop Prod, 5(4), 36-41.
Kachonpadungkitti, Y., & Jala, A. (2015). Influence of BA, IAA, 2,4-D and Kinetin on Micropropagation of Hylocereus undatus from Segments of Hypocotyl and True Leaf. Thai Journal of Science and Technology, 4(2), 147-154. (in Thai)
Karhu, S., & Hakala, K. (2007). Micropropagated Strawberries on the field. Acta Horticulturae, 567, 321-324.
Kaveeta, L., Nakhorn, N.M., Suwanwong, S., Tantiwiwat, S., & Wongkantharakorn, N. (2013). Plant physiology.
(3rd ed.). Bangkok: Kasetsart University. (in Thai)
Khan, S., Naz, S., Ali, K., & Zaidi, S. (2006). Direct organogenesis of Kalanchoe tomentosa (Crassulaceae)
from shoot tip. Pakistan Journal of Botany, 38(4), 977-981.
Madeeyah, N., Peeya, R., & Sahoh, S. (2017). Effects of Cytokynins and Concentrations on Development and Number of Multiple Shoots of in vitro Culture of Caladium bicolor Vent. YRU Journal of Science and Technology, 2(2), 57-65. (in Thai)
Manasseri, S. (2008). Planting Gloxinia in the soilless system. Research report, Faculty of Agricultural Technology, Songkhla Rajabhat University. (in Thai)
Meretia, M., Grigoriadoua, K., & Nanos, G. D. (2002). Micropropagation of the strawberry tree, Arbutus unedo L. Scientia Horticulturae, 93(2), 143-148.
Ministry of Agriculture and Cooperatives. (2003). Horticulture Tissue Technology. Bangkok: The Agricultural Cooperative Federation of Thailand. Limited. (in Thai)
Moradi, K., Otroshy, M., & Azimi, M.R. (2011). Micropropagation of strawberry by multiple shoots regeneration tissue cultures. Journal of Agricultural Technology, 7(6), 1755-1763.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
Neera, S., Soonthorn, W., Mongkolpun, P., & Hongpukdee, P. (2014). Effect of cytokinins and coconut water on in vitro shoot induction of Kaentawan (Helianthus tuberous L.). Khon kaen Agricultural Journal, 42(3), 328-334. (in Thai)
Phantong, P. (2017). Comparison of growth and physiological responses of Globba marantina L.and G. schomburgkii Hook. f. in growing substrate and hydroponic systems. Master of Science, Program in Environmental Biology, Faculty of Science, Suranaree University of Technology, Nakonratchasima. (in Thai)
Phetkhong, S., Sumochitraporn, S., & Makkasab, C. (2010). Surface sterlization study and growth regulators
effect in tissue culture of aquatic plant Cryptocoryne albida. In technical paper No.9/2010. Pathumthani: Aquaculture animal genetics Research and Development Institute. (in Thai)
Phromkhun, A. (2001). Propagation of 4 varieties of strawberries of In Vitro Culture. Master of Science,
Program in Agricultural Technolog, Faculty of Science, Valaya Alongkorn Rajabhat University, Pathumthani. (in Thai)
Rency, A.S., Pandian, S., & Ramesh, M. (2018). Influence of adenine sulphate on multiple shoot induction in Clitoria ternatea L. and analysis of phyto-compounds in in vitro grown plants. Biocatalysis and Agricultural Biotechnology, 16, 181-191.
Samransuk, Y. (2002). The classification of strawberry varieties by DNA analysis method. Master of Science, Program in Biotechnology, Faculty of Science, Chiang Mai University, Chiang Mai. (in Thai)
Taghavi, S., Barac, T., Greenberg, B., Borremans, B., Vangronsveld, J., & Lelie, D. (2005). Horizontal Gene Transfer to Endogenous Endophytic Bacteria from Poplar Improves Phytoremediation of Toluene. Applied and environmental microbiology, 71(12), 8500-8505.
Techapinyawat, S. (2005). Plant physiology. Bangkok : Kasetsart University. (in Thai)
Thongaram, D. (2004). Hydroponics. Ratchaburi : Thammarak Printing. (in Thai)
Treftz, C., & Omaye, S.T. (2015). Comparision between hydroponic and soil systems for growing strawberries
in a greenhouse. International Journal of Agriculture Extension, 3(3), 195-200.
U-Kong, W., Chundet, R., & Buddharak, P. (2012). In vitro culture of Momordica charantia L. Thai Agriculture Research Journa, 30(2), 198-206. (in Thai)
Zayed, A., Lytle, C. M., Qian, J. Hong., & Terry, N. (1998). Chromium accumulation, translocation and
chemical speciation in vegetable crops. Planta, 206, 293-299
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2019-09-30
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