Total Phenolic Compounds and Antioxidant Activity of Vegetable Herb and Fruit Juices in Na-o Sub-district Municipality, Meuang District, Loei Province
Abstract
The purpose of this research were: 1) to investigate the total phenolic contents and antioxidant activity of vegetable, herb and fruit juices and 2) to develop of functional beverages from vegetable herb and fruit Juices. Total phenolic contents of sample were Folin-Ciocaltue phenol method. The antioxidant activity of sample were assay by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and Ferric reducing/antioxidant potential (FRAP) assay. The sensory evaluation of sample was performed by using 5-point hedonic scale. Data were analyzed by mean and standard deviation. One-Way ANOVA were employed for analyzing comparison. And by pair to Fisher's least significant difference (LSD). Pearson correlation were employed for analyzing correlation. Our results showed that Psidium guajava and 4 formula exhibited higher total phenolic contents than the other vegetable herb fruit and formulas with at 30.21±1.88 and 29.31±2.05 µg GAE/ml sample, respectively. Citrus aurantifolia and 4 formula exhibited higher antioxidant activity than the other vegetable herb fruit and formulas expressed as DPPH assay value was 58.24±0.21 and 57.09±0.31 µM VEAC/ml sample, respectively. Psidium guajava and 4 formula exhibited higher antioxidant activity than the other vegetable herb fruit and formulas expressed as FRAP assay value was 65.20±1.17 and 59.57±0.81 µM VEAC/ml sample, respectively. Total phenolic contents of samples were significantly correlated with antioxidant activity. In addition, tests the difference of mean by pair of color, odor and overall acceptance found that the functional beverages of 4 formula had difference with 1 and 2 formulas with statistically significant at 0.05 level. This study suggested that trend for developing functional beverages from vegetables, herbs and fruits. Keywords : total phenolic content ; antioxidant activity ; vegetable herb and fruit JuicesReferences
Bamidele, O.P., & Fasogbon, M.B. (2017). Chemical and antioxidant properties of snake tomato (Trichosanthes cucumerina) juice and Pineapple (Ananas comosus) juice blends and their changes during storage. Food Chem, 220, 184 – 189.
Burakorn, J. & Praphruet, R. (2012). Antibacterial Activities of Seven Indigenous Vegetables. (2012).
Journal of Thai Traditional & Alternative Medicine, 10(1), 11-22. (in Thai)
Chanchay, N. (2011). Study of Carotenoid and Antioxidation Characteristic As a Side With The Northern
Food Lab. In Proceeding the 2nd MJU-Phrae National Research Conference. (pp. 834-837).
Phrae : Maejo University Phrae Campus. (in Thai)
Chen, Y.Y., Liu, J.F., Chen, C.M., Chao, P.Y., & Chang, T.J. (2003). A study of the antioxidative and antimutagenic effects of Houttuynia cordata Thunb. using an oxidized frying oil-fed model.
J Nutr Sci Vitaminol (Tokyo), 49(5), 327-33.
Ferri, L.A., Alves-Do-Prado, W., Yamada, S.S., Gazola, S., Batista, M.R., & Bazotte, R.B. (2006).
Investigation of the antihypertensive effect of oral crude stevioside in patients with mild essential hypertension. Phytother Res., 20(9), 732 – 736.
Fongthep N, Yodrak P, & Thaipong K. (2015). Fruit quality and antioxidant contents of different fruit grades in ‘Paen Seethong’ guava. Agricultural Sci J.,46(3)(Suppl.), 505-508 (in Thai)
Halee, A., & Rattanapun, B. (2017). Study of Antioxidant Efficacies of 15 Local Herbs. Kmutt Research and Development Journal, 40(2), 283-293. (in Thai)
Jandaruang, J., Siritapetawee, J., Thumanu, K., Songsiriritthigul, C., Krittanai, C., Daduang, S., (2012).
The Effects of Temperature and pH on Secondary Structure and Antioxidant Activity of Crocodylus siamensis Hemoglobin. Protein J., 31, 43 – 50.
Jumroon, A., Youryon, P. & Joyroy, B. (2015). Analysis of Antioxidant Activity in Musa (ABB group) ‘Kluai Hin’ and Musa (AA group) ‘Kluai Leb Mu Nang’. Songklanakarin Journal of Plant Science, 2(1), 38 – 42.
Katisart, T. & Surapong Rattana, S. (2017). Hypoglycemic Activity of Leaf Extracts from Tiliacora triandra in Normal and Streptozotocin-Induced Diabetic Rats. Pharmacognosy J., 5 , 621-625.
Kor-arnan, S., Paoblake, S., & Aswachaisuvikom, T. (2015). Antibacterial, Antioxidation, Antiproteolytic, and Cytotoxicity Activity of Stevia rebaudiana Bertoni Leaves. Journal of Science and Technology, Ubon Ratchathani University, 17(3), 8-55. (in Thai)
Kumchuen, E. (2014). Induction of program cell death and anti-metastatic activities of Houttuynia cordata Thunb. Extracts in human cervical cancer cell (thesis). Department of Clinical Chemistry, Faculty of Allied Health Science. Chulalongkorn University. p. 1-85. (in Thai)
Kumar, M., Satyendra, K. P., Sairam, K. & Siva, H. (2014). Antihyperglycemic Activity of Houttuynia cordata Thunb. in Streptozotocin-Induced Diabetic Rats. Advances in Pharmacological Sciences, 2014,1-12.
Kunnaja, P., Wongpalee, S.P., & Panthong, A. (2014). Evaluation of anti-inflammatory, analgesic, and antipyretic activities of the ethanol extract from Murdannia loriformis (Hassk.) Rolla Rao et Kammathy. Bioimpacts., 4(4), 183 – 189.
Oonsivilai R, Oonmetta-aree J, & Singthong J. (2011). Bioactivity and functional properties of Yanang, Krueo Manoy and Rang Chuet extracts. Nakhonratchasima: Suranaree University of Technology. Report No. SUT3-305-53-12-24. p. 1-53. (in Thai)
Paitoonyothin, T. & Weerasilp, P. (2007). Study of radical scavenging properties of Thai fruits (special project). Faculty of Pharmacy. Mahidol University. (in Thai)
Pakdeechote, P., Kukongviriyapan, U. & Kukongviriyapan, V. (2003). Screening for free radical scavenging activities of extracts from Coccinia grandis and Centella asiatica. Srinagarind Med J.,18(2), 78-84. (in Thai)
Plangsom, K. & Kanjanavas, P. (2015). Antibacterial activity of ethanolic extracts of Houttuynia cordata Thunb., Allium sativum and Amomum krervanh Pierre on some bacteria. Huachiew Chalermprakiet Science and Technology Journal, 1(2), 56-65.
Prasitpuriprecha, C., Damkliang, A., Surintha, P. & Deelum, W. (2009). Immunomodulating, Antioxidant and Antimicrobial Activities of Northeastern Thai Edible Plant and Medicinal Plant Extracts. IJPS, 5(2),
99 - 107. (in Thai)
Sae-tan, S. & Kunpanya, P. (2017). Water extract from leaf and stem of White Mugwort inhibits enzyme activity of D-amylase and D-glucosidase. Journal of Nutrition Asscociation of Thailand, 52(2),16-22. (in Thai)
Schulze, M.B., Manson, J.E., Ludwig, D.S., Colditz, G.A., Stampfer, M.J., Willett, W.C., et al. (2004). Sugar-Sweetened beverages, weight gain, and incidence of type2diabetes in young and middle- aged women. JAMA, 292 (8) , 927 – 934.
Shian, Ee T., Abdullah, A., Musa, K. H., Maskat, M. Y., & Ghani, M. A. (2012). Antioxidant properties of three banana cultivars (Musa acuminate ‘Berangan’, ‘Mas’ and ‘Raja’) extracts. Sain Malaysiana, 41(3) , 319 – 324.
Srimoon, R. & Tachai, S. (2014). Total phenolic compounds and free radicals scavenging content in processing fruit juice in Chanthaburi province. Rajamangala University of Technology Tawan-ok Research Journal, 7(1), 24-30. (in Thai)
Sripanlom, N. & Chidthong, R. (2004). Determination of Antioxidant Activity in Vegetables in Nakhon Pathom Municipality. Rajabhat Institute Nakhon Pathorn. p. 1-78. (in Thai)
Suchao-in, T. & Nitaramorn, P. (2010). Antioxidant vegetable pills (special project). Faculty of pharmacy. Bangkok: Mahidol University. (in Thai)
Subphala, T. (2008). Comparative studies among Murdannia bracteata, M. Loriformis and M. Nudiflora (Commelinaceae) in Thailand (thesis). Bangkok: Khon Kean University. (in Thai)
Taew-Teing, S. (2009). Soy milk product mixed with carrot juice (thesis). Department of Foods and Nutrition, Faculty of Home Economics Technology. Bangkok: Rajamangala University of Technology Phra Nakhon. p. 1-125. (in Thai)
Tararungruang, S. & Nawachartkul, N. (2012). Development of beverage from lycopene-containing vegetables/fruits/herbs (special project. Faculty of Pharmacy. Mahidol University. (in Thai)
Thonginla, V., Wanwimolruk, C., & Chuaybamroong, P. (2014). Correlation between antioxidant capacities of fruits analyzed with DMPD and phenolic contents, vitamin c, vitamin e and beta-carotene. Burapha Science Journal, 19 (2), 93-104. (in Thai)
Thongtha, S., Sawai, P., & Srisook, K. (2017). A comparative study on antioxidant and nitric oxide-inducing activity of some watermelon cultivars grown in Thailand. Burapha Science Journal., 22(special volume) National Science Research Conference 9th:14-22. (in Thai)
Tungtrakanpoung R, & Tungtrakanpoung J. (2016). Antibacterial activity from leaf extracts of Mentha cordifolia Opiz. by ethanol extraction. KKU Sci. J., 44(1), 79-87. (in Thai)
Wang, W., Zu, Y., Fu, Y., & Efferth, T. (2012). In vitroantioxidant and antimicrobial activity of extracts from Morus alba L. leaves, stems and fruits. J Chin Med, 40( 2), 349 – 356.
Wang, Y., Xiang, L., Wang, C., Tang, C. & He, X. (2013). Antidiabetic andantioxidant effects and phytochemical of mulberry fruit (Morus alba L.) polyphenol enhanced extract. Public Library of Science J, 8(7), 1 – 10.
Worarakulwong, T. & Wongsawadwech, S. (2012). Antimutagenicity and antioxidation activities of Artemisia vulgaris var. indica (special project). Faculty of pharmacy. Bangkok: Mahidol University. (in Thai)
Burakorn, J. & Praphruet, R. (2012). Antibacterial Activities of Seven Indigenous Vegetables. (2012).
Journal of Thai Traditional & Alternative Medicine, 10(1), 11-22. (in Thai)
Chanchay, N. (2011). Study of Carotenoid and Antioxidation Characteristic As a Side With The Northern
Food Lab. In Proceeding the 2nd MJU-Phrae National Research Conference. (pp. 834-837).
Phrae : Maejo University Phrae Campus. (in Thai)
Chen, Y.Y., Liu, J.F., Chen, C.M., Chao, P.Y., & Chang, T.J. (2003). A study of the antioxidative and antimutagenic effects of Houttuynia cordata Thunb. using an oxidized frying oil-fed model.
J Nutr Sci Vitaminol (Tokyo), 49(5), 327-33.
Ferri, L.A., Alves-Do-Prado, W., Yamada, S.S., Gazola, S., Batista, M.R., & Bazotte, R.B. (2006).
Investigation of the antihypertensive effect of oral crude stevioside in patients with mild essential hypertension. Phytother Res., 20(9), 732 – 736.
Fongthep N, Yodrak P, & Thaipong K. (2015). Fruit quality and antioxidant contents of different fruit grades in ‘Paen Seethong’ guava. Agricultural Sci J.,46(3)(Suppl.), 505-508 (in Thai)
Halee, A., & Rattanapun, B. (2017). Study of Antioxidant Efficacies of 15 Local Herbs. Kmutt Research and Development Journal, 40(2), 283-293. (in Thai)
Jandaruang, J., Siritapetawee, J., Thumanu, K., Songsiriritthigul, C., Krittanai, C., Daduang, S., (2012).
The Effects of Temperature and pH on Secondary Structure and Antioxidant Activity of Crocodylus siamensis Hemoglobin. Protein J., 31, 43 – 50.
Jumroon, A., Youryon, P. & Joyroy, B. (2015). Analysis of Antioxidant Activity in Musa (ABB group) ‘Kluai Hin’ and Musa (AA group) ‘Kluai Leb Mu Nang’. Songklanakarin Journal of Plant Science, 2(1), 38 – 42.
Katisart, T. & Surapong Rattana, S. (2017). Hypoglycemic Activity of Leaf Extracts from Tiliacora triandra in Normal and Streptozotocin-Induced Diabetic Rats. Pharmacognosy J., 5 , 621-625.
Kor-arnan, S., Paoblake, S., & Aswachaisuvikom, T. (2015). Antibacterial, Antioxidation, Antiproteolytic, and Cytotoxicity Activity of Stevia rebaudiana Bertoni Leaves. Journal of Science and Technology, Ubon Ratchathani University, 17(3), 8-55. (in Thai)
Kumchuen, E. (2014). Induction of program cell death and anti-metastatic activities of Houttuynia cordata Thunb. Extracts in human cervical cancer cell (thesis). Department of Clinical Chemistry, Faculty of Allied Health Science. Chulalongkorn University. p. 1-85. (in Thai)
Kumar, M., Satyendra, K. P., Sairam, K. & Siva, H. (2014). Antihyperglycemic Activity of Houttuynia cordata Thunb. in Streptozotocin-Induced Diabetic Rats. Advances in Pharmacological Sciences, 2014,1-12.
Kunnaja, P., Wongpalee, S.P., & Panthong, A. (2014). Evaluation of anti-inflammatory, analgesic, and antipyretic activities of the ethanol extract from Murdannia loriformis (Hassk.) Rolla Rao et Kammathy. Bioimpacts., 4(4), 183 – 189.
Oonsivilai R, Oonmetta-aree J, & Singthong J. (2011). Bioactivity and functional properties of Yanang, Krueo Manoy and Rang Chuet extracts. Nakhonratchasima: Suranaree University of Technology. Report No. SUT3-305-53-12-24. p. 1-53. (in Thai)
Paitoonyothin, T. & Weerasilp, P. (2007). Study of radical scavenging properties of Thai fruits (special project). Faculty of Pharmacy. Mahidol University. (in Thai)
Pakdeechote, P., Kukongviriyapan, U. & Kukongviriyapan, V. (2003). Screening for free radical scavenging activities of extracts from Coccinia grandis and Centella asiatica. Srinagarind Med J.,18(2), 78-84. (in Thai)
Plangsom, K. & Kanjanavas, P. (2015). Antibacterial activity of ethanolic extracts of Houttuynia cordata Thunb., Allium sativum and Amomum krervanh Pierre on some bacteria. Huachiew Chalermprakiet Science and Technology Journal, 1(2), 56-65.
Prasitpuriprecha, C., Damkliang, A., Surintha, P. & Deelum, W. (2009). Immunomodulating, Antioxidant and Antimicrobial Activities of Northeastern Thai Edible Plant and Medicinal Plant Extracts. IJPS, 5(2),
99 - 107. (in Thai)
Sae-tan, S. & Kunpanya, P. (2017). Water extract from leaf and stem of White Mugwort inhibits enzyme activity of D-amylase and D-glucosidase. Journal of Nutrition Asscociation of Thailand, 52(2),16-22. (in Thai)
Schulze, M.B., Manson, J.E., Ludwig, D.S., Colditz, G.A., Stampfer, M.J., Willett, W.C., et al. (2004). Sugar-Sweetened beverages, weight gain, and incidence of type2diabetes in young and middle- aged women. JAMA, 292 (8) , 927 – 934.
Shian, Ee T., Abdullah, A., Musa, K. H., Maskat, M. Y., & Ghani, M. A. (2012). Antioxidant properties of three banana cultivars (Musa acuminate ‘Berangan’, ‘Mas’ and ‘Raja’) extracts. Sain Malaysiana, 41(3) , 319 – 324.
Srimoon, R. & Tachai, S. (2014). Total phenolic compounds and free radicals scavenging content in processing fruit juice in Chanthaburi province. Rajamangala University of Technology Tawan-ok Research Journal, 7(1), 24-30. (in Thai)
Sripanlom, N. & Chidthong, R. (2004). Determination of Antioxidant Activity in Vegetables in Nakhon Pathom Municipality. Rajabhat Institute Nakhon Pathorn. p. 1-78. (in Thai)
Suchao-in, T. & Nitaramorn, P. (2010). Antioxidant vegetable pills (special project). Faculty of pharmacy. Bangkok: Mahidol University. (in Thai)
Subphala, T. (2008). Comparative studies among Murdannia bracteata, M. Loriformis and M. Nudiflora (Commelinaceae) in Thailand (thesis). Bangkok: Khon Kean University. (in Thai)
Taew-Teing, S. (2009). Soy milk product mixed with carrot juice (thesis). Department of Foods and Nutrition, Faculty of Home Economics Technology. Bangkok: Rajamangala University of Technology Phra Nakhon. p. 1-125. (in Thai)
Tararungruang, S. & Nawachartkul, N. (2012). Development of beverage from lycopene-containing vegetables/fruits/herbs (special project. Faculty of Pharmacy. Mahidol University. (in Thai)
Thonginla, V., Wanwimolruk, C., & Chuaybamroong, P. (2014). Correlation between antioxidant capacities of fruits analyzed with DMPD and phenolic contents, vitamin c, vitamin e and beta-carotene. Burapha Science Journal, 19 (2), 93-104. (in Thai)
Thongtha, S., Sawai, P., & Srisook, K. (2017). A comparative study on antioxidant and nitric oxide-inducing activity of some watermelon cultivars grown in Thailand. Burapha Science Journal., 22(special volume) National Science Research Conference 9th:14-22. (in Thai)
Tungtrakanpoung R, & Tungtrakanpoung J. (2016). Antibacterial activity from leaf extracts of Mentha cordifolia Opiz. by ethanol extraction. KKU Sci. J., 44(1), 79-87. (in Thai)
Wang, W., Zu, Y., Fu, Y., & Efferth, T. (2012). In vitroantioxidant and antimicrobial activity of extracts from Morus alba L. leaves, stems and fruits. J Chin Med, 40( 2), 349 – 356.
Wang, Y., Xiang, L., Wang, C., Tang, C. & He, X. (2013). Antidiabetic andantioxidant effects and phytochemical of mulberry fruit (Morus alba L.) polyphenol enhanced extract. Public Library of Science J, 8(7), 1 – 10.
Worarakulwong, T. & Wongsawadwech, S. (2012). Antimutagenicity and antioxidation activities of Artemisia vulgaris var. indica (special project). Faculty of pharmacy. Bangkok: Mahidol University. (in Thai)
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2020-09-01
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