Ethanol Production from Rubber Sawdust Waste by Thermotolerant Scheffersomyces shehatae TTC79
Abstract
Lignocellulosic materials are the most abundant, cheap and renewable feedstocks for ethanol production. In this study, rubber sawdust, a lignocellulosic waste of furniture manufacturers was investigated as a source of fermentable sugars for ethanol production. Enzyme hydrolysis of alkaline pretreated sawdust showed high sugar content and low amount of toxic compounds compared with the acid pretreated sawdust. Simultaneous saccharification and fermentation (SSF) studies were carried out to produce ethanol from alkaline pretreated sawdust waste using thermotolerant yeast, Scheffersomyces shehatae TTC79. The maximum ethanol concentration, ethanol yield, productivity and theoretical yield were 2.14 g/l, 0.33 g/g, 0.18 g/l/h and 65.13%, respectively. Keywords : ethanol, rubber sawdust, thermotolerant yeast, simultaneous saccharification and fermentationReferences
Auesukaree, C., Koedrith, P., Saenpayavai, P., Asvarak, T., Benjaphokee, S., Sugiyama, M., Kaneko, Y.,
Harashima, S. and Boonchird, C. (2012). Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits. Journal of Bioscience and Bioengineering, 114(2), 144-149.
Choudhary, J., Singh, S. and Nain, L. (2016). Thermotolerant fermenting yeasts for simultaneous
saccharification fermentation of lignocellulosic biomass. Electronic Journal of Biotechnology,
21, 82-92.
Govumoni, S. P., Koti, S., Kothagouni, S. Y., Venkateshwar, S. and Linga, V. R. (2013). Evaluation of
pretreatment methods for enzymatic saccharification of wheat straw for bioethanol production. Carbohydrate Polymers, 91(2), 646-650.
Jonsson, L. J., and Martín, C. (2016). Pretreatment of lignocellulose: formation of inhibitory by-products and
strategies for minimizing their effects. Bioresource Technology, 199, 103-112.
Kim, I., Lee, I., Jeon, S. H., Hwang, T., and Han, J. I. (2015). Hydrodynamic cavitation as a novel pretreatment
approach for bioethanol production from reed. Bioresource Technology, 192, 335-339.
Liu, Y., Zhang, G., Sun, H., Sun, X., Jiang, N., Rasool, A., Lin, Z. and Li, C. (2014). Enhanced pathway
efficiency of Saccharomyces cerevisiae by introducing thermo-tolerant devices. Bioresource Technology, 170, 38-44.
Maurya, D. P., Singla, A., and Negi, S. (2015). An overview of key pretreatment processes for biological
conversion of lignocellulosic biomass to bioethanol. 3 Biotech, 5(5), 597-609.
Mussatto, S. I., Machado, E. M., Carneiro, L. M., and Teixeira, J. A. (2012). Sugars metabolism and ethanol
production by different yeast strains from coffee industry wastes hydrolysates. Applied Energy,
92, 763-768.
Nopphakao, C., Yuvadetkun, P., Keawlor, N., Thamnasrirangkul, T., Poobudcha, Y. and Boonmee, M. (2014).
Evaluation of agricultural wastes for the use in ethanol production by Candida shehatae TISTR 5843. KKU Research Journal, 12, 11-18.
Oseghale, C.I. and Sideso, M.M. (2011). Effect of Temperature Variation on the Production of Ethanol from
Sawdust. Research Journal of Applied Sciences, 6(3), 205-208.
Senatham, S., Chamduang, T., Kaewchingduang, Y., Thammasittirong, A., Srisodsuk, M., Elliston, A.,
Roberts, L., Waldron, K. and Thammasittirong, N.S. (2016). Enhanced xylose fermentation and hydrolysate inhibitor tolerance of Scheffersomyces shehatae for efficient ethanol production from non-detoxified lignocellulosic hydrolysate. SpringerPlus. 5, 1040.
Shahsavarani, H., Hasegawa, D., Yokota, D., Sugiyama, M., Kaneko, Y., Boonchird, C. and Harashima, S.
(2013). Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14. Journal of Bioscience and Bioengineering, 115(1), 20-23.
Singh, A., and Bishnoi, N. R. (2013). Comparative study of various pretreatment techniques for ethanol
production from water hyacinth. Industrial Crops and Products, 44, 283-289.
Zheng, Y., Pan, Z., and Zhang, R. (2009). Overview of biomass pretreatment for cellulosic ethanol
production. International Journal of Agricultural and Biological Engineering, 2(3), 51-68.
Zheng, Y., Zhao, J., Xu, F., and Li, Y. (2014). Pretreatment of lignocellulosic biomass for enhanced biogas
production. Progress in Energy and Combustion Science, 42, 35-53.
Harashima, S. and Boonchird, C. (2012). Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits. Journal of Bioscience and Bioengineering, 114(2), 144-149.
Choudhary, J., Singh, S. and Nain, L. (2016). Thermotolerant fermenting yeasts for simultaneous
saccharification fermentation of lignocellulosic biomass. Electronic Journal of Biotechnology,
21, 82-92.
Govumoni, S. P., Koti, S., Kothagouni, S. Y., Venkateshwar, S. and Linga, V. R. (2013). Evaluation of
pretreatment methods for enzymatic saccharification of wheat straw for bioethanol production. Carbohydrate Polymers, 91(2), 646-650.
Jonsson, L. J., and Martín, C. (2016). Pretreatment of lignocellulose: formation of inhibitory by-products and
strategies for minimizing their effects. Bioresource Technology, 199, 103-112.
Kim, I., Lee, I., Jeon, S. H., Hwang, T., and Han, J. I. (2015). Hydrodynamic cavitation as a novel pretreatment
approach for bioethanol production from reed. Bioresource Technology, 192, 335-339.
Liu, Y., Zhang, G., Sun, H., Sun, X., Jiang, N., Rasool, A., Lin, Z. and Li, C. (2014). Enhanced pathway
efficiency of Saccharomyces cerevisiae by introducing thermo-tolerant devices. Bioresource Technology, 170, 38-44.
Maurya, D. P., Singla, A., and Negi, S. (2015). An overview of key pretreatment processes for biological
conversion of lignocellulosic biomass to bioethanol. 3 Biotech, 5(5), 597-609.
Mussatto, S. I., Machado, E. M., Carneiro, L. M., and Teixeira, J. A. (2012). Sugars metabolism and ethanol
production by different yeast strains from coffee industry wastes hydrolysates. Applied Energy,
92, 763-768.
Nopphakao, C., Yuvadetkun, P., Keawlor, N., Thamnasrirangkul, T., Poobudcha, Y. and Boonmee, M. (2014).
Evaluation of agricultural wastes for the use in ethanol production by Candida shehatae TISTR 5843. KKU Research Journal, 12, 11-18.
Oseghale, C.I. and Sideso, M.M. (2011). Effect of Temperature Variation on the Production of Ethanol from
Sawdust. Research Journal of Applied Sciences, 6(3), 205-208.
Senatham, S., Chamduang, T., Kaewchingduang, Y., Thammasittirong, A., Srisodsuk, M., Elliston, A.,
Roberts, L., Waldron, K. and Thammasittirong, N.S. (2016). Enhanced xylose fermentation and hydrolysate inhibitor tolerance of Scheffersomyces shehatae for efficient ethanol production from non-detoxified lignocellulosic hydrolysate. SpringerPlus. 5, 1040.
Shahsavarani, H., Hasegawa, D., Yokota, D., Sugiyama, M., Kaneko, Y., Boonchird, C. and Harashima, S.
(2013). Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14. Journal of Bioscience and Bioengineering, 115(1), 20-23.
Singh, A., and Bishnoi, N. R. (2013). Comparative study of various pretreatment techniques for ethanol
production from water hyacinth. Industrial Crops and Products, 44, 283-289.
Zheng, Y., Pan, Z., and Zhang, R. (2009). Overview of biomass pretreatment for cellulosic ethanol
production. International Journal of Agricultural and Biological Engineering, 2(3), 51-68.
Zheng, Y., Zhao, J., Xu, F., and Li, Y. (2014). Pretreatment of lignocellulosic biomass for enhanced biogas
production. Progress in Energy and Combustion Science, 42, 35-53.
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Published
2017-08-07
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 9
