Effect of Plant Growth Regulators on In Vitro Propagation of Dendrobium ellipsophyllum Tang & F.T.Wang
Abstract
In vitro seedlings of Dendrobium ellipsophyllum Tang & F.T.Wang were cultured on MS medium supplemented with cytokinin (BA, kinetin, TDZ) at 0 0.1 0.5 1.0 or 2.0 mg/L for 12 weeks. The result showed that the highest percentage of shoot formation (47.5%), shoot numbers (1.85 shoots/plant) and leaf numbers (4.45 leaves/plant) were obtained when cultured on the medium with 0.1 mg/L TDZ. D. ellipsophyllum Tang & F.T.Wang seedling on medium was supplemented with auxin (NAA, IAA, IBA) at 0 0.1 0.5 1.0 or 2.0 mg/L for 12 weeks. The result showed that the highest percentage of shoot formation (42.5%), shoot numbers (1.73 shoots/plant) including highest of root numbers (4.40 roots/plant) (100%) was observed on the medium supplemented with 2.0 mg/L NAA. Moreover, the seedling of D. ellipsophyllum Tang & F.T.Wang on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L TDZ for 12 weeks. The results showed the highest percentage of shoot formation (50%), the highest average of shoot numbers (1.90 shoots/plant) and leaf numbers (5.68 leaves/plant).Keyword: Dendrobium ellipsophyllum Tang & F.T.Wang, plant growth regulators, in vitroReferences
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(Dendrobium nobile) var. Emma White. African Journal of Biotechnology, 10(16), 3097 – 3103.
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Chawla, H.S. (2002). Introduction to plant biotechnology. (2nd ed.) USA: Science Publishers.
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Chen, Y., Goodale, U.M., Fan, X.L. and Gao, J.Y. (2015). Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum an orchid with an extremely small population in China. Global Ecology and Conservation, 3, 367 – 378.
Choopeng, S. (2015). Effect of plant growth regulators on growth and development of in vitro Phaius tankervilleae (Banks ex L’ Hér.) Blume. Songklanakarin Journal of Plant Science, 2(2), 32 – 35.
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Duncan, D. B. (1955). Multiple range and multiple F test. Biometrics, 11, 1 – 42.
Evans, D.E., Coleman, J.O.D. and Kearns, A. (2003). Plant cell culture. London: Bios Scientific Publishers.
George, E.F. and Sherrington, P.D. (1984). Plant propagation by tissue culture: Handbook and directory of commercial laboratories. Basingstoke, England: Exegetics.
Hajong, S., Kumaria S. and Tandon P. (2013). Effect of plant growth regulators on regeneration potential of axenic nodal segments of Dendrobium chrysanthum Wall. ex Lindl. Journal of Agricultural Science and Technology, 15, 1425 – 1435.
Kijwijan, B. (2004). Technology Introduction of Plant Tissue Culture for Plant Breeding. Khon Kaen: Klangnanavittaya Press. (in Thai)
Knudson, L. (1946). A new nutrient for the germination of orchid seeds. American Orchid Society Bulletin, 15, 214 – 217.
Kulpa, D. and Katron, J. (2012). Seed germination and plant development of Bletilla striata in vitro. Folia Pomeranae Universitatis Technologiae Stetinensis, 293(21), 51 – 60.
Mahendran, G. and Bai, V.N. (2015). Mass propagation of Satyrium nepalense D.Don. a medicinal orchid via seed culture. Scientia Horticulturae, 119, 203 – 207.
Mary, S.S and Divakar, K.M. (2015). In vitro propagation of epiphytic orchid Pholidota imbricata Hook. of Western Ghats. International Journal of Engineering Research & Technology, 4(10), 107 – 112.
Marknoi, C. (2013). Flora at Phu Suan Sai . Chiang Mai: The Botanical Garden Organization, Ministry of Natural Resources and Environment. (in Thai)
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology, 15, 473 – 497.
Nahar, S.J., Kazuhiko, S. and Haque, S.M. (2012). Effect of polysaccharides including elicitors on organogenesis in protocorm-like body (PLB) of Cymbidium insigne in vitro. Journal of Agricultural Science and Technology, 2, 1029 – 1033.
Naing, A.H., Park, I.S., Hwang, Y.J., Chung, J.D. and Lim, K.B. (2010). In vitro micropropagation and conservation of Rhynchostylis retusa BL. Horticulture, Environment, and Biotechnology, 51(5),
440 – 444.
Parvin, M.S., Haque, M.E., Akhter, F., Moniruzzaman. and Khaldun, A.B.M. (2009). Effect of different levels of NAA on in vitro growth and development of shoots of Dendrobium orchid. Bangladesh Journal of Agricultural Research, 34(3), 411 – 416.
Prasertsongskun, S. (2006). Plant Tissue Culture for Plant Breeding. Bangkok: Forepace Publishing House. (in Thai)
Razdan, M.K. (2002). Introduction to plant tissue culture. (2nd ed.) USA: Science Publishers.
Ritti, W., Chourykaew, B., Kitcharoen, T. and Nontarak, K. (2015). Effects of auxins and cytokinins on growth and development of in vitro seedling culture of Dendrobium formosum Roxb. ex Lindl.
In Proceedings of the 9th Botanical Conference of Thailand. (pp. 128 – 138). Department of Botany, Faculty of Science, Chulalongkorn University. (in Thai)
Ritti, W., Chourykaew, B., Phrombangyuan, P. and Thaksin, S. (2016). Effect of chitosan on growth and development of in vitro seedling of Panisea uniflora (Lindl.) Lindl. Songklanakarin Journal of Plant Science, 3(4), 8 – 13. (in Thai)
Sangchanjiradet, S and Thangthong, J. (2015). In vitro seed culture of Dendrobium delacourii Guillaumin. SDU Research Journal, 8(1), 109 – 118. (in Thai)
Santarunai, N. (2015). Effect of medium and plant growth regulator on development and flowering
(Dendrobium friedericksianum Rchb.f.) of orchid in vitro. In The National Conference & Research
Presentation 2015 “Create and Development to Approach ASEAN Community II”. (pp. 155 – 162).
Nakhon Ratchasima College. (in Thai)
Sittisujjatham, S. (2006). Wild Orchid of Thailand (6th ed). Bangkok: Baan Lae Suan Printing. (in Thai)
Tao, J., Yu, L., Kong, F and Zhao, D. (2011). Effects of plant growth regulators on in vitro propagation of Cymbidium faberi Rolfe. African Journal of Biotechnology, 10(69), 15639 – 15646.
Thaithong, O. (2008). Thai orchids. (15th.) Bangkok: Baan Lae Suan Printing. (in Thai)
Tokaew, W., Chantaranothai, P. and Phonsena, P. (2015). Orchids in Nam Nao National Park. Khon Kaen: Klangnanavittaya Press. (in Thai)
Visittavanit, A. and Tantiwiwat, S. (2009). Effect of cytokinins and banana on growth and development of Dendrobium cariniferum Rchb.f. in vitro culture. In Proceedings of 47th Kasetsart University Annual Conference: Plants. (pp. 1 - 7). Kasetsart University. (in Thai)
(Dendrobium nobile) var. Emma White. African Journal of Biotechnology, 10(16), 3097 – 3103.
Bhattacharyya, P., Kmaria, S. and Tandon, P. (2016). High frequency regeneration protocol for Dendrobium nobile a model tissue culture approach for propagation of medicinally important orchid species. South African Journal of Botany, 104, 232 – 243.
Chawla, H.S. (2002). Introduction to plant biotechnology. (2nd ed.) USA: Science Publishers.
Chen, J.T. and Chang, W.C. (2000). Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae). Plant Science, 160, 87– 93.
Chen, Y., Goodale, U.M., Fan, X.L. and Gao, J.Y. (2015). Asymbiotic seed germination and in vitro seedling development of Paphiopedilum spicerianum an orchid with an extremely small population in China. Global Ecology and Conservation, 3, 367 – 378.
Choopeng, S. (2015). Effect of plant growth regulators on growth and development of in vitro Phaius tankervilleae (Banks ex L’ Hér.) Blume. Songklanakarin Journal of Plant Science, 2(2), 32 – 35.
(in Thai)
Duncan, D. B. (1955). Multiple range and multiple F test. Biometrics, 11, 1 – 42.
Evans, D.E., Coleman, J.O.D. and Kearns, A. (2003). Plant cell culture. London: Bios Scientific Publishers.
George, E.F. and Sherrington, P.D. (1984). Plant propagation by tissue culture: Handbook and directory of commercial laboratories. Basingstoke, England: Exegetics.
Hajong, S., Kumaria S. and Tandon P. (2013). Effect of plant growth regulators on regeneration potential of axenic nodal segments of Dendrobium chrysanthum Wall. ex Lindl. Journal of Agricultural Science and Technology, 15, 1425 – 1435.
Kijwijan, B. (2004). Technology Introduction of Plant Tissue Culture for Plant Breeding. Khon Kaen: Klangnanavittaya Press. (in Thai)
Knudson, L. (1946). A new nutrient for the germination of orchid seeds. American Orchid Society Bulletin, 15, 214 – 217.
Kulpa, D. and Katron, J. (2012). Seed germination and plant development of Bletilla striata in vitro. Folia Pomeranae Universitatis Technologiae Stetinensis, 293(21), 51 – 60.
Mahendran, G. and Bai, V.N. (2015). Mass propagation of Satyrium nepalense D.Don. a medicinal orchid via seed culture. Scientia Horticulturae, 119, 203 – 207.
Mary, S.S and Divakar, K.M. (2015). In vitro propagation of epiphytic orchid Pholidota imbricata Hook. of Western Ghats. International Journal of Engineering Research & Technology, 4(10), 107 – 112.
Marknoi, C. (2013). Flora at Phu Suan Sai . Chiang Mai: The Botanical Garden Organization, Ministry of Natural Resources and Environment. (in Thai)
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiology, 15, 473 – 497.
Nahar, S.J., Kazuhiko, S. and Haque, S.M. (2012). Effect of polysaccharides including elicitors on organogenesis in protocorm-like body (PLB) of Cymbidium insigne in vitro. Journal of Agricultural Science and Technology, 2, 1029 – 1033.
Naing, A.H., Park, I.S., Hwang, Y.J., Chung, J.D. and Lim, K.B. (2010). In vitro micropropagation and conservation of Rhynchostylis retusa BL. Horticulture, Environment, and Biotechnology, 51(5),
440 – 444.
Parvin, M.S., Haque, M.E., Akhter, F., Moniruzzaman. and Khaldun, A.B.M. (2009). Effect of different levels of NAA on in vitro growth and development of shoots of Dendrobium orchid. Bangladesh Journal of Agricultural Research, 34(3), 411 – 416.
Prasertsongskun, S. (2006). Plant Tissue Culture for Plant Breeding. Bangkok: Forepace Publishing House. (in Thai)
Razdan, M.K. (2002). Introduction to plant tissue culture. (2nd ed.) USA: Science Publishers.
Ritti, W., Chourykaew, B., Kitcharoen, T. and Nontarak, K. (2015). Effects of auxins and cytokinins on growth and development of in vitro seedling culture of Dendrobium formosum Roxb. ex Lindl.
In Proceedings of the 9th Botanical Conference of Thailand. (pp. 128 – 138). Department of Botany, Faculty of Science, Chulalongkorn University. (in Thai)
Ritti, W., Chourykaew, B., Phrombangyuan, P. and Thaksin, S. (2016). Effect of chitosan on growth and development of in vitro seedling of Panisea uniflora (Lindl.) Lindl. Songklanakarin Journal of Plant Science, 3(4), 8 – 13. (in Thai)
Sangchanjiradet, S and Thangthong, J. (2015). In vitro seed culture of Dendrobium delacourii Guillaumin. SDU Research Journal, 8(1), 109 – 118. (in Thai)
Santarunai, N. (2015). Effect of medium and plant growth regulator on development and flowering
(Dendrobium friedericksianum Rchb.f.) of orchid in vitro. In The National Conference & Research
Presentation 2015 “Create and Development to Approach ASEAN Community II”. (pp. 155 – 162).
Nakhon Ratchasima College. (in Thai)
Sittisujjatham, S. (2006). Wild Orchid of Thailand (6th ed). Bangkok: Baan Lae Suan Printing. (in Thai)
Tao, J., Yu, L., Kong, F and Zhao, D. (2011). Effects of plant growth regulators on in vitro propagation of Cymbidium faberi Rolfe. African Journal of Biotechnology, 10(69), 15639 – 15646.
Thaithong, O. (2008). Thai orchids. (15th.) Bangkok: Baan Lae Suan Printing. (in Thai)
Tokaew, W., Chantaranothai, P. and Phonsena, P. (2015). Orchids in Nam Nao National Park. Khon Kaen: Klangnanavittaya Press. (in Thai)
Visittavanit, A. and Tantiwiwat, S. (2009). Effect of cytokinins and banana on growth and development of Dendrobium cariniferum Rchb.f. in vitro culture. In Proceedings of 47th Kasetsart University Annual Conference: Plants. (pp. 1 - 7). Kasetsart University. (in Thai)
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2017-07-06
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