Adverse Effects of Abamectin on Hematological Profile and Histological Alterations of Hybrid Catfish (Clarias macrocephalus x C. gariepinus)
Abstract
The study was designed to investigate the adverse effects of abamectin concentration of 5 µl L-1 for 0, 5, 10, 15 and 20 days on the hematological profile and histological alterations of hybrid catfish (Clarias macrocephalus x C.gariepinus). It was found that the exposure time influenced the content of hemoglobin concentration percentage (Hb gm (%)), red blood cell (RBC), and white blood cell (WBC) counts. The percentage of Hb and RBC count significantly decreased (p<0.05) after juvenile hybrid catfish exposed to abamectin for 15 and 20 days compared to the control group. Furthermore, an increasing in exposure time resulted in the significant increase (p<0.05) of WBC count. The leukocyte was found only 3 types: lymphocyte, monocyte and neutrophil. Histological alterations in gill, intestine, liver and kidney illustrated the changes of tissue depending on the time of exposure. There were deformation of secondary lamellae and necrosis of gills. The intestine showed increasing of goblet cells and lesion, and the liver illustrated necrosis of hepatocytes. In addition, necrosis of the tubular cell and glomerulus deformation were found in kidney. In conclusion, hybrid catfish could be useful to evaluate the toxicity of abamectin contaminated in aquatic environment by studying its hematological profile and histological alterations. Keywords : abamectin, hematology, hybrid catfish, leukocyte, toxicityReferences
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gill, liver and gut tissue of mosquitofish, Gambusia affinis: A microscopic study. Environmental Toxicology and Pharmacology, 21 (3), 246-253.
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Singh, D., Nath, K., Trivedi, S. P. & Sharma, Y. K. (2008). Impact of copper on haematological profile of freshwater fish, Channa punctatus. Journal of Fish Biology, 29, 253-257.
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Thanomsit, C. (2016). Evaluation of abamectin effect on some biochemical constituents and
histological alterations in Asian sea bass (Lates calcarifer). Naresuan university Journal : Science and Technology, 24 (1), 72-81.
Thanomsit, C., Wattanakornsiri, A. & Nanthanawat, P. (2016). Effect of Glyphosate on fish behavior and
histological alteration of gills in Asian sea bass (Lates calcarifer). Burapha Science Journal, 21 (2),
204-215.
Thophon, S. K., Kruatrachue, M., Upathan, E. S., Pokthitiyook, P., Sahaphong, S. & Jaritkhuan, S. (2003).
Histophatological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure. Environmental Pollution, 121, 307-320.
Valenzeula, A., Oyarzŭn, C. & Silva, V. (2003). Blood cells of the Schroederichtys xhlensis (Guichenot, 1848):
the leukocytes (Elasmobranchil, Scyliorhinidae). Gayana, 67, 130-137.
Vázquez, G. R. & Guerrero, G. A. (2007). Characterization of blood cells and hematological parameter in Cichlasoma dimerus (Teleostei, Perciformes). Tissue and Cell, 39, 151-160.
Wedemeyer, G. A. & Yasutake, W. T. (1997). Clinical methods for the assessment of the effects of environmental stress on fish health. Technical papers No. 89. U.S. Department of the Interior, Fish and wildlife service; Washington, D. C.
Yancheva, V., Velcheva, I., Stoyanova, S. & Georgieva, E. (2016). Histological biomarkers in fish as a
tool in ecological risk assessment and monitoring programs: A review. Applied Ecology and
Environmental Research, 14 (1), 47-75.
Ali, A. J., Khan, A., Khaliq, T., Javed, I., Muhammad, F., Aslam, B. & Khan, M. Z. (2013). Hematobiochemical disruptions by lambda-cyhalothrin in rats. The Pakistan Veterinary Journal, 34, 54-57.
Barber, D.L., Westermann, J. E. M. & White, M. G. (1981). The blood cells of the Antartic icefish Chaenocephalus aceratus Lónnberg: light and electron microscopic observations. Journal of Fish Biology, 19, 11-28.
Blaxhall, P. C. & Daisley, K. W. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology, 5, 771-781.
Cengiz, E. I. & ünlu, E. (2006). Sublethal effects of commercial deltamethrin on the structure of the
gill, liver and gut tissue of mosquitofish, Gambusia affinis: A microscopic study. Environmental Toxicology and Pharmacology, 21 (3), 246-253.
El-Said, M. M. (2007). Evaluation of Abamectin toxicity on some biochemical constituents
and osmoregulation in freshwater fish Oreochromis niloticus (Tilapia niloticus). Journal of the Egyptian Society of Toxicology, 37, 1-10.
Flores-Lopes, F. & Thomaz, A. T. (2011). Histopathologic alterations observed in fish gills as a tool in
environmental monitoring. Brazilian Journal of Biology, 1 (71), 179-188.
Ghaffar, A., Ashraf, S., Hussain, R., Hussain, T., Shafique, M., Noreen, S. & Aslam, S. (2014). Clinico-
Hematological Disparities Induced by Triazophos (Organophosphate) in Japanese quail. Pakistan
Vertinary Journal, 34, 257-259.
Groff, J. M. & Zinkl, J. G. (1999). Hematology and clinical chemistry of Cyprinid fish, Common carp and
goldfish. Veterinary Clinics of North America: Exotic Animal Practice, 2, 741-746.
Hasan, Z., Ghayyur, S., Hassan, U. & Rafique, S. (2015). Histomorphometric and Hematological Profile of Grass Carp (Ctenopharyngodon idella) during Acute Endosulfan Toxicity. The Pakistan Veterinary Journal, 35, 23-37.
Hinton, D. E., Baumann, P. C., Gardner, G. R., Hawkins, W. E., Hendricks, J. D., Murchelano, R. A. &
Okihiro, M. S. (1992). Histopathologic biomarkers. Biochemical, physiological, and histological markers of anthropogenic stress. In: Biomaker. Lewis Publisher: Boca Raton, pp.155-209.
Hrubec, T. C., Cardinale, J. L. & Smith, S. A. (2000). Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Veterinary Clinical Pathology, 29, 7-12.
Jenčič, V., Cerne, M., Erzen, N. K., Kobal, S. & Cerkvenik-Flajs, V. (2006). Abamectin effects on rainbow trout (Oncorhynchus mykiss). Ecotoxicology, 15, 249-257.
Martin, R. (1997). Modes of action of anthelmintic drugs. The Veterinary Journal, 154, 11-34.
Mohamed, F. A. S. (2009). Histopathlogical studies on Tilapia zillii and Solea vulgaris from Lake Qarum, Egypt.
World Journal of Fish and Marine Science, 1 (1), 29-39.
Oliveira Ribeiro, C. A., Vollaire, Y., Sanchez-Chardi, A. & Roche, H. (2005). Bioaccumulation and the effects of
organochlorine pesticides, PAH and heavy metals in the eel (Anguilla anguilla) at the Camargue Nature Reserve, France. Aquatic Toxicology, 74, 53-69.
Osman, A. G. M., Abd El Reheem, A. M. & AbuelFadi, K. Y. (2010). Enzymatic and histopathologic
biomarker as indicators of aquatic pollution in fish. Natural Science, 2 (11), 1302-1311.
Pamila, D., Subbaiyan, P. A. & Ramaswamy, M. (1991). Toxic effect of chromium and cobalt on Sartherodon mossambicus (peters). Indian journal of environmental health, 33, 218-224.
Raina, S. & Sachar, A. (2014). Effect of Heavy Metal, Zinc and Carbamate Pesticide, Sevin on Haematological Parameters of Fish, Labeo boga. The International Journal of Innovative Research in Science, Engineering and Technology, 3, 12636-12644.
Roth, M., Richards, R. H. & Sommerville, C. (1993). Current practices in the chemotherapeutic control of sea lice infestations in aquaculture: a review. Journal of Fish Diseases, 16, 1-26.
Singh, D., Nath, K., Trivedi, S. P. & Sharma, Y. K. (2008). Impact of copper on haematological profile of freshwater fish, Channa punctatus. Journal of Fish Biology, 29, 253-257.
Tavares-Dias, M. & Barcellos, J. F. M. (2005). Peripheral blood cells of the armored catfish, Hoplosternum littorale Hancock, 1828: a morphological and cytochemical study. The Brazilian Journal of Pharmaceutical Sciences, 22, 215-220.
Thanomsit, C. (2016). Evaluation of abamectin effect on some biochemical constituents and
histological alterations in Asian sea bass (Lates calcarifer). Naresuan university Journal : Science and Technology, 24 (1), 72-81.
Thanomsit, C., Wattanakornsiri, A. & Nanthanawat, P. (2016). Effect of Glyphosate on fish behavior and
histological alteration of gills in Asian sea bass (Lates calcarifer). Burapha Science Journal, 21 (2),
204-215.
Thophon, S. K., Kruatrachue, M., Upathan, E. S., Pokthitiyook, P., Sahaphong, S. & Jaritkhuan, S. (2003).
Histophatological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure. Environmental Pollution, 121, 307-320.
Valenzeula, A., Oyarzŭn, C. & Silva, V. (2003). Blood cells of the Schroederichtys xhlensis (Guichenot, 1848):
the leukocytes (Elasmobranchil, Scyliorhinidae). Gayana, 67, 130-137.
Vázquez, G. R. & Guerrero, G. A. (2007). Characterization of blood cells and hematological parameter in Cichlasoma dimerus (Teleostei, Perciformes). Tissue and Cell, 39, 151-160.
Wedemeyer, G. A. & Yasutake, W. T. (1997). Clinical methods for the assessment of the effects of environmental stress on fish health. Technical papers No. 89. U.S. Department of the Interior, Fish and wildlife service; Washington, D. C.
Yancheva, V., Velcheva, I., Stoyanova, S. & Georgieva, E. (2016). Histological biomarkers in fish as a
tool in ecological risk assessment and monitoring programs: A review. Applied Ecology and
Environmental Research, 14 (1), 47-75.
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2017-06-19
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