Screening of Carotenogenic Yeasts from Natural Soil at the University of Phayao
Abstract
This study aimed to isolate carotenogenic yeasts from natural soil at the University of Phayao. Three isolates of yeasts displayed orange or pink colonies were obtained after screening on Potato dextrose agar (PDA) plates, which were named as UP11 UP12 and UPF2. Internal transcribed spacer (ITS)-based identification and similarity search upon GenBank database highly specified UP12 and UPF2 as Rhodotorula mucilaginosa. In contrast, the taxonomic position of UP11 was obscured since there was no reasonable matching sequence in the database. This observation suggested that alternative molecular chronometer should be taken into consideration. The optimum temperature for carotenoid production for both UP11 and UP12 was 30 oC, which were announced as 28.59±1.69 and 14.59±0.55 µg.g-1 cell dry weight, respectively. Keywords : carotenoid, carotenogenic yeast, University of PhayaoReferences
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El-Banna, A. A., El-Razek, A. M. A., & El-Mahdy, A. R. (2012). Isolation, identification and screening of carotenoid-production strains of Rhodotorula glutinis. Food and Nutrition Sciences, 3, 627-633.
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Peerier, V., Dubreucq, E., & Gayzy, P. (1995). Fatty acid and carotenoid composition of Rhodotorula strains. Archives of Microbiology, 164, 173-179
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White, T.J., Bruns, T., Lee, S., & Taylor J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M.A. Innis, D.H. Gelfand. J.J. Sninsky and T.J. White. (Eds.), PCR protocols- A guide to methods and applications. (pp. 315-322). New York: Academic Press, Inc.
Yurkov, A. M., Vustin, M. M., Tyaglov, B. V., Maksimova, I. A., & Sinekoiy S. P. (2008). Pigmented basidiomycetous yeasts are a promising source of carotenoids and ubiquinone Q10. Microbiology,
77(1), 1–6.
Blaalid, R., Kumar, S., Nilsson, R. H., Abarenkov, K., Kirks, P. M., & Kahserud, H. (2013). ITS1 versus ITS2 as DNA metabarcodes for fungi. Molecular Ecology Resources. 13, 218–224.
El-Banna, A. A., El-Razek, A. M. A., & El-Mahdy, A. R. (2012). Isolation, identification and screening of carotenoid-production strains of Rhodotorula glutinis. Food and Nutrition Sciences, 3, 627-633.
Guedes, A. C., Amaro, H. M., & Maleata, F. X. (2011). Microalgae as sources of carotenoids. Marine Drugs, 9,
625 – 644.
Maldonade. I. R., Scamparini, A. R. P., & Rodriguez-Amaya, D. B. (2007). Selection and characterization of carotenoid-producing yeasts from Campinas region, Brazil. Brazillian Journal of Microbiology, 38, 65-70.
Marova, I., Certik, M., & Breierova, E. (2011). Production of enriched biomass by carotenogenic yeasts- Application of whole cell yeast biomass to production of pigments and other lipid compound. In D. Matovic. (Ed.), Biomass - Detection, Production and Usage, (pp. 345-384). Shanghai: In Tech China.
Naghavi, F. S., Hanachi, P., & Saboora, A. (2014). Effect of temperature, pH and salinity on carotenoid production in Rodotorula mucilaginosa. Research in Biotechnology, 5(4), 01-04.
Peerier, V., Dubreucq, E., & Gayzy, P. (1995). Fatty acid and carotenoid composition of Rhodotorula strains. Archives of Microbiology, 164, 173-179
Pewlong, W. & Bangwirunruk, J. (2009). Increasing the carotenoid content of Xanthophyllomyces dendrorhous by a neutron-ultraviolet combination treatment. In Proceeding Conference on Nuclear Science and Technology. (pp. 1-10). Bangkok: Thailand. (in Thai)
Prakash, B. B. (2001). Studies on yeast Rhodotorula, its carotenoids and their applications. Thesis, Doctor of Philosophy. University of Pune. India.
Tansin, K. (2010). Enhancement of carotenoid production by Phaffia rhodozyma and its stability. Thesis, Master of Science, Silpakorn University, Thailand. (in Thai)
White, T.J., Bruns, T., Lee, S., & Taylor J. W. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M.A. Innis, D.H. Gelfand. J.J. Sninsky and T.J. White. (Eds.), PCR protocols- A guide to methods and applications. (pp. 315-322). New York: Academic Press, Inc.
Yurkov, A. M., Vustin, M. M., Tyaglov, B. V., Maksimova, I. A., & Sinekoiy S. P. (2008). Pigmented basidiomycetous yeasts are a promising source of carotenoids and ubiquinone Q10. Microbiology,
77(1), 1–6.
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2016-09-09
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บทความวิจัยจากการประชุมวิชาการระดับชาติ"วิทยาศาสตร์วิจัย"ครั้งที่ 8
