Toxicity Evaluation of Glyphosate on Mortality Rate and Histological Alteration in Black Rice Crab (Sayarmia sp.)
Abstract
This research aimed to study the toxicity of glyphosate on black rice crab (Sayarmia spp.) by assessing the mortality rates and alterations in gill tissue and hepatopancreas. The crab was exposed to glyphosate in different concentrations: 0.02, 0.2, 2 and 20 ml L-1 of water mixed within 1 kg of clay from rice paddy. After exposed, mortality rates at 96 h were 20, 80 and 100% at the concentrations of 0.2, 2 and 20 ml L-1, consecutively. The LC50 values of black rice crab at 24, 48, 72 and 96 h after exposure were 25.98, 12.55, 3.65 and 0.97 ml L-1, respectively. The alterations in gill tissue occurred were edema and infiltration of haemocytes; in addition, there were distended lumen, damaged myoepithelial layer, large vacuole and necrosis in hepatopancreas. These alterations depended on exposure time and glyphosate concentration. Based on the results, it concluded that glyphosate adversely affected on black rice crab in both mortality rate and tissue alteration; thus, the black rice crab can be used as biomonitor for glyphosate contamination in the aquatic organism. Keywords : LC50, probit analysis, glyphosate, black rice crab, histologyReferences
Andhale, A. V., Bhosale, P. A. & Zambare, S. P. (2011). Histopathological study of nickel induced alterations in
the fresh water bivalve, Lammellidens marginalis. Journal of Experimental Sciences, 2(4), 1-3.
Arellano, J. M., Storch, V. & Sarasquete, C. (2004). Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. Journal of Applied Ichthyology, 20, 452–460.
Ayanda, O. I. & Egbamuno, E. A. (2012). Histological examination of the liver and gills of Clarias gariepinus treated with glyphosate, Environmental Research Journal, 6 (3), 228-234.
Braz-Mota, S., Sadaus -Henrique., H., Duarte, R. M., Val, A. L., & Almeida-Val, V. M.F. (2015). Roundup exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macroponum. Chemosphere, 135, 53-60.
Bodhipaksha, N. & Weeks Perkins, B. A. (1994). The effects of methyl parathion on phagocytosis and respiratory burst activity of tiger shrimp (Penaeus monodon) phagocytosis. In: Stolen, J.S., Fletcher, T.C. (Eds.), Modulators of Fish Immune Responses, Models for Environmental Toxicology Biomarkers Immunostimulators, vol. 1. SOS Publication, Fair Haven, N. J. pp.11–12.
Borggaard, O. K. & Gimsing, A. L. (2008). Fate of glyphosate in soil and the possibility of leaching to ground and surface waters: a review. Pest Management Science, 64, 441-456.
Chourpagar, A. R. & Kulkarni, G. K. (2013). Impact of copper sulphate on gill structure of a freshwater female crab, Barytelphusa cunicularis (Westwood). Journal of Academia and Industrial Research (JAIR), 2(3). 167-169.
Giesy, J. P., Dobson, S. & Solomon, K. R. (2000). Ecotoxicological risk assessment for Roundup herbicide. Reviews of Environmental Contamination and Toxicology, 167, 35-120.
Harayashiki, C. A. Y., Varela Junior, A. S., Souza Machado, A. A., Costa Cabrera, L., Primel., E. G., Bianchini, A. & Corcini, C. D. (2013). Toxic effects of the herbicide Roundup in the guppy Poecilia vivipara acclimated to freshwater. Aquatic Toxicology, 142-143, 176-184.
Jaykumar, S. (2002). Effect of Copper and Zinc toxicity on freshwater crab, Spiralothelphusa hydrozoma. Research Journal of Agriculture and Bilogical Science. 14(6), 775-781.
Jiraungkoorskul, W., Upathama, S., Kruatrachuea, M., Sahaphongc, S., Vichasri-Gramsa, S. & Pokethitiyook, P. (2002). Histopathological effects of Roundup, a glyphosate herbicide, on Nile tilapia (Oreochromis niloticus). ScienceAsia, 28, 121-127.
Konstantinou, I. K., Hela, D. G. & Albanis, T. A. (2006). The status of pesticide pollution in surface waters (rivers and lakes) of Greece. Environmental Pollution, 141(3), 555-570.
Kumar, S. & Tembhre, M. (2010). Fish and Fisheries. New Central agencies (P) Ltd, London. Maharajan, A ., Narayanasamy, Y., Ganapiriya, V. & Shanmugavel, K. (2015). Histological alterations of a combination of Chlorpyrifos and Cypermethrin (Nurocombi) insecticide in the fresh water crab, Paratelphusa jacquemontii (Rathbun). The Journal of Basic and Applied Zoology, 72, 104-112.
Maharajan, A., Narayanasamy, Y., Ganapiriya, V. & Shanmugavei, K. (2015). Histological alterations of a combination of Chlorpyrifos and Cypermetrin (Nurocombi) insecticide in the freshwater crab, Paratelphusa jacquemontii (Rathbun). The journal of Basic & Applied Zoology, 72, 104-112.
Moreno, N. C., Sofia, S. H. & Martinez, C. B. R. (2014). Genotoxic effects of the herbicide Roundup Transorb® and its active ingredient glyphosate on the fish Prochilodus lineatus. Environmental Toxicology and Pharmacology, 37, 448–454.
Office of - Agricultural Economics (OAE). (2010). Summary of imported pesticides. Retrieved April 20, 2015,
from http://www.oae.go.th.
Office of - Agriculture Regulation (OAR). (2010). Public access database. Retrieved April 10, 2015 from http://m.doa.go.th.
Oliveira Ribeiro, C. .A., Vollaire, Y., Sanchez-Chardi, A. & Roche, H. (2005). Bioaccumulation and the effects of organochlorine pesticides, PAH and heavy metals in the eel (Anguilla anguilla) at the Camargue Nature Reserve, France. Aquatic Toxicology, 74, 53–69.
Panuwet, P., Siriwong, W., Prapamontol, T., Ryan, P. B. Fiedler, N., Robsone, M. G. & Barr, D. B. (2012). Agricultural Pesticide management in Thailand: status and population health risk. Environmental Science and Policy, 17, 72-81.
Pachanawan, A. & Compuch, S. (2006). Effect of sex on growth and molting of black crab ( Somaniathelphusa germaini). Journal of Fisheries Technology Research, 2 (1), 88-92. (In Thai)
Siddon, C. E. & Witman, J. D. (2004). Behavioral indirect interactions: multiple predator effects and prey
switching in the rocky subtidal, Ecology, 85, 2938-2945.
Sprague, J. B. (1971). The measurement of pollutant toxicity to fish, sub lethal effect and safe concentration. Water Research Journal, 5, 245-266.
Stentiford, G. D. & Feist, S. W. (2005). A histopathological survey of shore crab (Carcinus maenas) and brown shrimp (Crangon crangon) from six estuaries in the United Kingdom. Journal of Invertebrate Pathology, 88, 136–146.
Shaikh, F. I., Ustad, I. R. & Ansari, N. T. (2010). Effect of heavy metal on the ovary of freshwater crab, Barytelphusa cunicularis (Westwood). Bioscan Journal, 5 (2), 335-338.
Thanomsit, C. (2016a). Evaluation of abamectin effect on some biochemical constituents and histological alterations in Asian sea bass (Lates calcarifer). Naresuan university Journal: Science and Technology, 24 (1), 72-8.
Thanomsit, C., Wattanakornsiri, A. & Nanthanawat, P. (2016b). Effect of glyphosate on fish behavior and histological alteration of gills in Asian sea bass (Lates calcarifer). Burapha Science Journal, 21 (2),
204-215.
Tiamkao, P. (2014). Glyphosate toxicity and microbial degradation. King Mongkut’s Agricultural Journal, 32 (3), 71-79. (In Thai)
Tungare, S. M. & Sawant, A. D. (2000). Physiological effects of heavy metals on prawns. International Conference on Probing in Biological System in Mumbai. p.139.
Vigliano, F. A., Aleman, N., Quiroga, M. I. & Nieto, J. M. (2006). Ultrastructural characterization of gills in juveniles of the Argentinian silverside, Odontesthes bonariensis (Valenciennes, 1835) (Teleostei: Atheriniformes). Anatomia Histologia Embryologia, 35, 76–83.
Walker, C. H., Hopkin, S. P., Sibly, R.M., and Peakall, D. B. (2006). Principle of Ecotoxicology. Taylor & Francis: USA.
World Health Organization (WHO). (1994). Glyphosate. Environmental Health Criteria, Plublication. Geneva. Switzerland.
Zouaoui, K., Dulaurent, S., Gaulier, J. M., Moesch, C. & Lachatre, G. (2013). Determination of glyphosate and AMPA in blood and urine from humans: about 13 cases of acute intoxication. Forensic Science International, 226, 20-25.
the fresh water bivalve, Lammellidens marginalis. Journal of Experimental Sciences, 2(4), 1-3.
Arellano, J. M., Storch, V. & Sarasquete, C. (2004). Ultrastructural and histochemical study on gills and skin of the Senegal sole, Solea senegalensis. Journal of Applied Ichthyology, 20, 452–460.
Ayanda, O. I. & Egbamuno, E. A. (2012). Histological examination of the liver and gills of Clarias gariepinus treated with glyphosate, Environmental Research Journal, 6 (3), 228-234.
Braz-Mota, S., Sadaus -Henrique., H., Duarte, R. M., Val, A. L., & Almeida-Val, V. M.F. (2015). Roundup exposure promotes gills and liver impairments, DNA damage and inhibition of brain cholinergic activity in the Amazon teleost fish Colossoma macroponum. Chemosphere, 135, 53-60.
Bodhipaksha, N. & Weeks Perkins, B. A. (1994). The effects of methyl parathion on phagocytosis and respiratory burst activity of tiger shrimp (Penaeus monodon) phagocytosis. In: Stolen, J.S., Fletcher, T.C. (Eds.), Modulators of Fish Immune Responses, Models for Environmental Toxicology Biomarkers Immunostimulators, vol. 1. SOS Publication, Fair Haven, N. J. pp.11–12.
Borggaard, O. K. & Gimsing, A. L. (2008). Fate of glyphosate in soil and the possibility of leaching to ground and surface waters: a review. Pest Management Science, 64, 441-456.
Chourpagar, A. R. & Kulkarni, G. K. (2013). Impact of copper sulphate on gill structure of a freshwater female crab, Barytelphusa cunicularis (Westwood). Journal of Academia and Industrial Research (JAIR), 2(3). 167-169.
Giesy, J. P., Dobson, S. & Solomon, K. R. (2000). Ecotoxicological risk assessment for Roundup herbicide. Reviews of Environmental Contamination and Toxicology, 167, 35-120.
Harayashiki, C. A. Y., Varela Junior, A. S., Souza Machado, A. A., Costa Cabrera, L., Primel., E. G., Bianchini, A. & Corcini, C. D. (2013). Toxic effects of the herbicide Roundup in the guppy Poecilia vivipara acclimated to freshwater. Aquatic Toxicology, 142-143, 176-184.
Jaykumar, S. (2002). Effect of Copper and Zinc toxicity on freshwater crab, Spiralothelphusa hydrozoma. Research Journal of Agriculture and Bilogical Science. 14(6), 775-781.
Jiraungkoorskul, W., Upathama, S., Kruatrachuea, M., Sahaphongc, S., Vichasri-Gramsa, S. & Pokethitiyook, P. (2002). Histopathological effects of Roundup, a glyphosate herbicide, on Nile tilapia (Oreochromis niloticus). ScienceAsia, 28, 121-127.
Konstantinou, I. K., Hela, D. G. & Albanis, T. A. (2006). The status of pesticide pollution in surface waters (rivers and lakes) of Greece. Environmental Pollution, 141(3), 555-570.
Kumar, S. & Tembhre, M. (2010). Fish and Fisheries. New Central agencies (P) Ltd, London. Maharajan, A ., Narayanasamy, Y., Ganapiriya, V. & Shanmugavel, K. (2015). Histological alterations of a combination of Chlorpyrifos and Cypermethrin (Nurocombi) insecticide in the fresh water crab, Paratelphusa jacquemontii (Rathbun). The Journal of Basic and Applied Zoology, 72, 104-112.
Maharajan, A., Narayanasamy, Y., Ganapiriya, V. & Shanmugavei, K. (2015). Histological alterations of a combination of Chlorpyrifos and Cypermetrin (Nurocombi) insecticide in the freshwater crab, Paratelphusa jacquemontii (Rathbun). The journal of Basic & Applied Zoology, 72, 104-112.
Moreno, N. C., Sofia, S. H. & Martinez, C. B. R. (2014). Genotoxic effects of the herbicide Roundup Transorb® and its active ingredient glyphosate on the fish Prochilodus lineatus. Environmental Toxicology and Pharmacology, 37, 448–454.
Office of - Agricultural Economics (OAE). (2010). Summary of imported pesticides. Retrieved April 20, 2015,
from http://www.oae.go.th.
Office of - Agriculture Regulation (OAR). (2010). Public access database. Retrieved April 10, 2015 from http://m.doa.go.th.
Oliveira Ribeiro, C. .A., Vollaire, Y., Sanchez-Chardi, A. & Roche, H. (2005). Bioaccumulation and the effects of organochlorine pesticides, PAH and heavy metals in the eel (Anguilla anguilla) at the Camargue Nature Reserve, France. Aquatic Toxicology, 74, 53–69.
Panuwet, P., Siriwong, W., Prapamontol, T., Ryan, P. B. Fiedler, N., Robsone, M. G. & Barr, D. B. (2012). Agricultural Pesticide management in Thailand: status and population health risk. Environmental Science and Policy, 17, 72-81.
Pachanawan, A. & Compuch, S. (2006). Effect of sex on growth and molting of black crab ( Somaniathelphusa germaini). Journal of Fisheries Technology Research, 2 (1), 88-92. (In Thai)
Siddon, C. E. & Witman, J. D. (2004). Behavioral indirect interactions: multiple predator effects and prey
switching in the rocky subtidal, Ecology, 85, 2938-2945.
Sprague, J. B. (1971). The measurement of pollutant toxicity to fish, sub lethal effect and safe concentration. Water Research Journal, 5, 245-266.
Stentiford, G. D. & Feist, S. W. (2005). A histopathological survey of shore crab (Carcinus maenas) and brown shrimp (Crangon crangon) from six estuaries in the United Kingdom. Journal of Invertebrate Pathology, 88, 136–146.
Shaikh, F. I., Ustad, I. R. & Ansari, N. T. (2010). Effect of heavy metal on the ovary of freshwater crab, Barytelphusa cunicularis (Westwood). Bioscan Journal, 5 (2), 335-338.
Thanomsit, C. (2016a). Evaluation of abamectin effect on some biochemical constituents and histological alterations in Asian sea bass (Lates calcarifer). Naresuan university Journal: Science and Technology, 24 (1), 72-8.
Thanomsit, C., Wattanakornsiri, A. & Nanthanawat, P. (2016b). Effect of glyphosate on fish behavior and histological alteration of gills in Asian sea bass (Lates calcarifer). Burapha Science Journal, 21 (2),
204-215.
Tiamkao, P. (2014). Glyphosate toxicity and microbial degradation. King Mongkut’s Agricultural Journal, 32 (3), 71-79. (In Thai)
Tungare, S. M. & Sawant, A. D. (2000). Physiological effects of heavy metals on prawns. International Conference on Probing in Biological System in Mumbai. p.139.
Vigliano, F. A., Aleman, N., Quiroga, M. I. & Nieto, J. M. (2006). Ultrastructural characterization of gills in juveniles of the Argentinian silverside, Odontesthes bonariensis (Valenciennes, 1835) (Teleostei: Atheriniformes). Anatomia Histologia Embryologia, 35, 76–83.
Walker, C. H., Hopkin, S. P., Sibly, R.M., and Peakall, D. B. (2006). Principle of Ecotoxicology. Taylor & Francis: USA.
World Health Organization (WHO). (1994). Glyphosate. Environmental Health Criteria, Plublication. Geneva. Switzerland.
Zouaoui, K., Dulaurent, S., Gaulier, J. M., Moesch, C. & Lachatre, G. (2013). Determination of glyphosate and AMPA in blood and urine from humans: about 13 cases of acute intoxication. Forensic Science International, 226, 20-25.
Downloads
Published
2016-10-13
Issue
Section
Research Article
